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将外源基因转染宿原代B淋巴细胞,可以对诸如腺苷脱氨酶(ADA)缺乏症等疾病产生直接的疗效,也因为基因转染的B淋巴细胞可以释放某些产物至血液中而对某些遗传病如血友病等产生一定的疗效。并且由于B淋巴细胞具有抗原性提呈能力,外源基因的转染可以增强机体对某些肿瘤及病毒的免疫反应。由于目前大多数将外源基因转染淋巴细胞的方法依赖于将这些细胞的体外扩增反药物筛选。而原代B淋巴细胞不易在体外长期培养,这使人们常不选择淋巴细胞作为基因转染的靶细胞。本研究克服了这一难题,建立了小鼠B淋巴细胞基因治疗模型。将携带有人ADA基因及Neo抗性基因的逆
Transfection of exogenous genes into primary B lymphocytes can have a direct effect on diseases such as adenosine deaminase (ADA) deficiency and because genetically-transfected B lymphocytes release certain products into the bloodstream And some genetic diseases such as hemophilia have a certain effect. And because B lymphocytes have antigenicity, exogenous gene transfection can enhance the body’s immune response to certain tumors and viruses. Since most current methods of transfection of foreign genes into lymphocytes rely on the in vitro amplification of these cells for anti-drug screening. The primary B lymphocytes are not easy to long-term culture in vitro, which makes people often do not choose lymphocytes as gene transfection target cells. This study overcomes this problem and establishes a mouse B lymphocyte gene therapy model. Will carry the reverse of the human ADA and Neo resistance genes