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目的研究不同免疫途径对马疫锥虫动基体DNA(kDNA)诱导正常小鼠产生抗双链DNA(dsDNA)抗体及其致病性的影响。方法将马疫锥虫kDNA与不完全弗氏佐剂乳化混合,通过皮下、腹腔、肌肉及静脉等途径注射入正常BALB/C小鼠。8周后,检测小鼠血清抗dsDNA抗体亚型及滴度、血尿素氮(BUN)、血肌酐(Scr)、补体C3、24h尿蛋白浓度、肾小球免疫复合物沉积强度、肾组织病变活动指数等,并分析肾组织病理学特征。结果上述各组小鼠产生IgG型抗dsDNA抗体量及肾损害程度依次为:皮下组>腹腔组>肌肉组>静脉组(P<0.05);IgG型抗dsDNA抗体与肾组织病变活动指数呈正相关。结论不同免疫途径对马疫锥虫kDNA诱导抗dsDNA抗体产生有明显不同影响,其导致的狼疮样肾脏损害与该抗体亚型有关。
Objective To study the effect of different immunization pathways on the production of anti-dsDNA antibodies and their pathogenicity in normal mouse induced by kT DNA. Methods The trypanosome kappa DNA was emulsified with incomplete Freund’s adjuvant and injected into normal BALB / C mice by subcutaneous, intraperitoneal, intramuscular and intravenous routes. After 8 weeks, the serum anti-dsDNA antibody subtype and titer, blood urea nitrogen (BUN), serum creatinine (Scr), complement C3, 24h urinary protein concentration, glomerular immune complex deposition intensity, renal tissue lesions Activity index, etc., and analysis of renal histopathological features. Results The quantity of anti-dsDNA antibody and the extent of renal damage in the mice in each group were as follows: subcutaneous group> abdominal cavity> muscle group> vein group (P <0.05); IgG anti-dsDNA antibody was positively correlated with renal tissue lesion activity index . Conclusion Different routes of immunization have distinctly different effects on the induction of anti-dsDNA antibody by kDNA of Trypanosoma tentatum, and the lupoid-like renal damage caused by it is related to the antibody subtype.