制作疏松结缔组织切片难度较大 ,组织块易酥脆、收缩 ,不易切片。笔者经过反复摸索 ,用ＡＳＡＦ液取代常用固定液 ,用乙醇 -正丁醇混合脱水剂取代乙醇 ,克服了上述不足 ,现将方法介绍如下 :ＡＳＡＦ液的配制 :取水杨酸 10ｇ溶于 85ｍｌ、95 %乙醇中 ,再加入纯醋酸 5ｍｌ、40 % Made of loose connective tissue sections more difficult, crisp tissue block, contraction, not easy to slice. After repeated exploration, the authors used ASAF liquid to replace the common fixative liquid and replaced the ethanol with ethanol-n-butanol mixed dehydrating agent to overcome the above shortcomings. The preparation of ASAF liquid was as follows: Dissolve salicylic acid 10g in 85ml, 95 % Ethanol, then add pure acetic acid 5ml, 40%