论文部分内容阅读
目的:探讨用大孔吸附树脂法富集丁香叶抗菌有效部位。方法:以静态吸附与解吸附实验对大孔吸附树脂进行筛选。选定D101树脂进行分离工艺优化,包括树脂用量、径高比、洗脱流速、洗脱液浓度及用量等。结果:结果表明,最佳的条件为55%乙醇洗脱,吸附流速1 BV·h~(-1),洗脱流速5 BV·h~(-1),6 BV25%乙醇去除杂质,8 BV的55%乙醇洗脱得到有效部位。结论:提取液经D101树脂纯化后抗菌有效成分的含量达到65%以上,表明本方法适用于大规模制备丁香叶抗菌有效部位。
Objective: To investigate the enrichment of antimicrobial effective sites of clove leaf by macroporous adsorption resin. Methods: Macroporous adsorption resin was screened by static adsorption and desorption experiments. Selected D101 resin separation process optimization, including the amount of resin, aspect ratio, elution flow rate, eluent concentration and dosage. Results: The optimum conditions were 55% ethanol, 1 BV · h -1, 5 BV · h -1, 6 BV25% ethanol, 8 BV Of 55% ethanol to obtain the effective site. CONCLUSION: The content of antimicrobial active ingredients in the extract after D101 resin purification reaches more than 65%, indicating that the method is suitable for large-scale preparation of antibacterial effective parts of clove leaf.