论文部分内容阅读
为广泛了解我县老、少、边、穷地区农村HBV的传染源及传播途径,1990年8月对不受天气变化影响的枯水季节作为生活饮用,同时又在一个水系中洗衣物、洗菜、游泳并受生活污水及人畜便污染的塘水,采集取水部位水样34份,取上清水样(必要时离心)2份按2+1缓慢加入用0.O2mol/lpH7.0Na_2HPO_1缓冲液配成39％(分子量为6000)的聚乙二醇使沉淀HBsAg;2000r/min离心,将上清液弃去空干,按1+200浓缩量加0.025mol/LpH5.8的乙酸缓冲液使沉淀溶解,采用ELISA快速法检测HBsAg,检出阳性水样2份,检出率为5.88％(P/N>2.1为阳性)。
In order to make a broad understanding of the source of infection and transmission of HBV in old rural areas, low rural areas and poor rural areas in China, in August 1990, drinking water was used for drinking in the dry season, which is not affected by the weather changes, while washing clothes in a water system Dishes, swimming and contaminated by domestic sewage and human and animal ponds, collecting water intake 34 parts of the water sample, take the supernatant water (centrifuge if necessary) 2 2 + 1 slowly added with 0. 2mol / lpH7.0Na_2HPO_1 buffer 39% (molecular weight 6000) of polyethylene glycol to precipitate HBsAg; 2000r / min centrifugation, the supernatant was discarded to air dry, according to 1 +200 concentration plus 0.025mol / LpH5.8 acetate buffer The precipitate was dissolved and HBsAg was detected by ELISA. Two positive samples were detected, the detection rate was 5.88% (P / N> 2.1 was positive).