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目的：研究、探讨血小板源生长因子和肝素对培养的人主动脉平滑肌细胞表型转变调节作用，及其对平滑肌细胞胶原蛋白合成的影响。方法：~３Ｈ－脯氨酸掺入，电镜观察。~３Ｈ－脯氨酸掺入的比较采用ｔ检验。结果：血小板源生长因子能促进培养的动脉平滑肌细胞向合成表型转变，促进平滑肌细胞胶原蛋白的合成与分泌。肝素能拮抗血小板源生长因子促进平滑肌细胞向合成表型的转变，抑制平滑肌细胞胶原蛋白的合成。结论：血小板源生长因子能通过促进动脉平滑肌细胞向合成表型转变促进了平滑肌细胞胶原蛋白的合成；肝素能通过桔抗血小板源生长因子的作用抑制平滑肌细胞胶原蛋白合成。
AIM: To investigate the regulatory effect of platelet-derived growth factor and heparin on cultured human aortic smooth muscle cells phenotypes and their effects on collagen synthesis in smooth muscle cells. Methods: ~ 3H-proline incorporation, electron microscopy. ~ 3H-proline incorporation using t test. Results: Platelet-derived growth factor (VEGF) promoted the transition of cultured aortic smooth muscle cells to synthetic phenotype and promoted the synthesis and secretion of collagen in smooth muscle cells. Heparin can antagonize platelet-derived growth factor to promote the smooth muscle cells to the synthesis of phenotype changes, inhibition of smooth muscle cell collagen synthesis. CONCLUSION: PDGF can promote the synthesis of collagen in smooth muscle cells by promoting the transition from arterial smooth muscle cells to synthetic phenotypes. Heparin inhibits the synthesis of collagen in smooth muscle cells through the action of anti-platelet-derived growth factor.