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目的:分析与评价麋鹿角中多糖类成分。方法:分别采用Elson-Morgan法、硫酸咔唑法及苯酚硫酸法,以氨基葡萄糖、葡萄糖醛酸和葡萄糖为对照品,测定不同鹿龄及不同角部位麋鹿角样品中的氨基多糖、酸性多糖及中性多糖,并与鹿角进行比较。结果:多糖类成分含量因鹿龄和角部位不同存在较大差异。3种多糖类成分总含量以2岁龄麋鹿角为最高,其次为5岁龄,6~8岁龄,3岁龄和4岁龄。不同鹿龄麋鹿角3个角部位平均含量均以中性多糖为最高,其次是氨基多糖,酸性多糖含量相对较低。3种多糖在3个角部位中的平均含量均以2岁龄麋鹿角为最高,其中氨基多糖和中性多糖的含量与宁夏产鹿角接近,酸性多糖低于宁夏产鹿角。结论:该研究可为麋鹿角药用资源的合理利用及其产业化提供科学依据。
Objective: To analyze and evaluate polysaccharides in elk horn. Methods: Elson-Morgan method, carbazole sulfate method and phenol-sulfuric acid method were used respectively. Glucosamine, glucuronic acid and glucose were used as reference materials to determine the content of amino-polysaccharides, Neutral polysaccharides, and compared with antlers. Results: The contents of polysaccharides were quite different due to different deer age and angle. The total content of the three kinds of polysaccharides was highest at 2-year-old elk angle, followed by 5 years old, 6 to 8 years old, 3 years old and 4 years old. The average content of the three horns of elk antler at different deer age were highest with neutral polysaccharide, followed by amino polysaccharide, and the content of acid polysaccharide was relatively low. The average content of three kinds of polysaccharides in the three horns were highest at the age of 2 years. The contents of amino-polysaccharides and neutral polysaccharides were close to those in Ningxia, and the content of acid polysaccharides was lower than that in Ningxia. Conclusion: This study may provide a scientific basis for the rational utilization of elk anthelmintic resources and their industrialization.