论文部分内容阅读
采用盆栽实验研究了生长调节物质水杨酸(SA)、茉莉酸甲酯(MeJA)、赤霉素(GA3)对不同生长时期丹参植株中非结构糖含量、碳/氮比及根中丹酚酸类物质积累的影响;并进一步测定培养基中不同浓度蔗糖、葡萄糖、果糖对丹参毛状根中丹酚酸类物质积累的影响,对盆栽实验的结论进行了验证。结果显示,SA处理的丹参幼苗及花后期植株中蔗糖含量有增加趋势,而MeJA处理的丹参幼苗及花后期植株及GA3处理的丹参花后期植株中蔗糖积累均有降低趋势;且SA、MeJA和GA3处理对花后期植株地上和地下部分碳/氮比的影响不同。然而,SA和MeJA处理的丹参幼苗及花后期植株地上部分和根中还原糖含量、GA3处理的花后期植株根中还原糖含量均显著增加;同时,SA和MeJA处理的丹参幼苗根中迷迭香酸含量,以及SA、MeJA、GA3处理的花后期植株根中迷迭香酸含量和丹酚酸类总量显著增加。毛状根培养结果进一步证明,葡萄糖促进毛状根中迷迭香酸的产生,增加丹酚酸类总量,毛状根中迷迭香酸、丹酚酸B的积累及丹酚酸类总量与培养基中蔗糖浓度不相关。可见,丹参(植株)根中丹酚酸类物质的产生和积累受SA、MeJA和GA3的诱导,其与碳/氮比及植株中蔗糖含量没有相关性,推测植株中葡萄糖含量的增加促进根中丹酚酸类物质的积累。
Pot experiments were conducted to study the effects of growth regulator Salicylic Acid (SA), MeJA and GA3 on the content of unstructural carbohydrate, C / N ratio, The effects of different concentrations of sucrose, glucose and fructose on the accumulation of salvianolic acids in hairy roots of Salvia miltiorrhiza were also determined. The conclusions of the pot experiment were also verified. The results showed that the content of sucrose in SA-treated Salvia miltiorrhiza seedlings and flower-stage plants tended to increase, while the accumulation of sucrose in MeJA-treated Salvia miltiorrhiza seedlings and late-flowering plants and GA3- The effect of GA3 treatment on the C / N ratio of aerial and underground parts of late-flowering plants was different. However, the content of reducing sugar in aerial parts and root of Salvia miltiorrhiza seedlings and in post-flowering plants treated with SA and MeJA, and the content of reducing sugars in roots of post-flowering plants treated with GA3 increased significantly. Meanwhile, SA and MeJA The content of rosmarinic acid and the total amount of salvianolic acids in the roots of the flower after SA, MeJA, GA3 treatment significantly increased. The results of hairy root culture further proved that glucose promoted the production of rosmarinic acid in hairy roots, increased the total amount of salvianolic acids, the accumulation of rosmarinic acid, salvianolic acid B and the total salvianolic acids in hairy roots The amount is not related to the sucrose concentration in the medium. It can be seen that the production and accumulation of salvianolic acids in roots of Salvia miltiorrhiza (plant) were induced by SA, MeJA and GA3, which had no correlation with C / N ratio and sucrose content in plants, it was speculated that the increase of glucose content in the plants promoted roots The accumulation of phenols in the middle of the Dan.