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目的:建立HPLC法测定小鼠血浆、脑、心脏、肝脏、脾脏、肺脏、肾脏及睾丸中的活性硫水平。方法:70只ICR小鼠随机分为对照组,低、中、高剂量雄黄组,甘草次酸对照组,甘草次酸低、高剂量干预组,每组10只,灌胃给药,每日1次,共染毒8周。采用HPLC法检测染毒后小鼠血浆、脑、心脏、肝脏、脾脏、肺脏、肾脏及睾丸组织中的活性硫含量。结果:与对照组相比,雄黄高剂量组血浆、脑、肝脏、肾脏中活性硫水平显著降低(P<0.05);与雄黄高剂量组相比,甘草次酸高剂量干预组血浆、脑、肝脏、肾脏的活性硫水平明显升高(P<0.05),甘草次酸低剂量干预组血浆的活性硫水平明显升高(P<0.05)。结论:该方法简便、准确,灵敏度高。甘草次酸对雄黄所引起的血浆、脑、肝脏、肾脏中活性硫水平的降低有明显的保护作用。
Objective: To establish an HPLC method for the determination of active sulfur in plasma, brain, heart, liver, spleen, lung, kidney and testis in mice. Methods: Seventy ICR mice were randomly divided into control group, low, medium and high dose realgar group, glycyrrhetinic acid control group, glycyrrhetinic acid low and high dose intervention group, 10 rats in each group. 1 times, co-infected for 8 weeks. The content of active sulfur in plasma, brain, heart, liver, spleen, lung, kidney and testis of mice was detected by HPLC. Results: Compared with the control group, the levels of active sulfur in plasma, brain, liver and kidney were significantly decreased in high-dose realgar group (P <0.05). Compared with the high-dose realgar group, The levels of active sulfur in liver and kidney were significantly increased (P <0.05), and the level of active sulfur in plasma was significantly increased in glycyrrhetinic acid low dose intervention group (P <0.05). Conclusion: The method is simple, accurate and sensitive. Glycyrrhetinic acid on Realgar caused by the plasma, brain, liver, kidney activity in the reduction of significant role in the protection of sulfur.