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目的探讨阿霉素对大鼠原代心肌细胞凋亡的影响,并探讨其作用机理。方法取培养72 h原代心肌细胞,随机分为空白对照组(C组)和阿霉素处理组(D组),空白对照组(C组)不给予任何药物、阿霉素组(D组)用1μmol/L阿霉素处理24 h。对原代心肌细胞的存活率进行6次测定,取平均值;采用免疫荧光法对心肌纯度进行检测;采用吸光光度法对两组的MTT值进行检测;采用ELISA法对两组的心肌肌钙蛋白(cTnI)和氨基末端B型利钠肽前体(NT-proBNP)的含量进行测定。结果原代心肌细胞6次测定的平均成活率为95.38%,免疫荧光法鉴定心肌纯度为82.25%。与C组相比,D组MTT值显著降低,cTnI值和NT-proBNP值显著升高,差异具有统计学意义(P<0.05)。结论阿霉素能够导致大鼠原代心肌细胞发生凋亡,具有明显的心脏毒性。
Objective To investigate the effect of doxorubicin on primary cardiomyocyte apoptosis in rats and its mechanism of action. Methods 72 h primary cultured cardiomyocytes were randomly divided into blank control group (C group) and doxorubicin treatment group (D group), blank control group (C group) without any drug, Doxorubicin group ) For 24 h with 1 μmol / L doxorubicin. The viability of primary cardiomyocytes was measured 6 times and averaged. The myocardial purity was detected by immunofluorescence method. The MTT values of both groups were determined by spectrophotometry. Protein (cTnI) and amino-terminal B-type natriuretic peptide precursor (NT-proBNP) were measured. Results The average viability of primary cardiomyocytes in 6 determinations was 95.38%. The myocardial purity was 82.25% by immunofluorescence. Compared with group C, the MTT in group D was significantly lower, and the values of cTnI and NT-proBNP were significantly higher in group D (P <0.05). Conclusion Adriamycin can induce primary cardiomyocyte apoptosis in rats with obvious cardiotoxicity.