论文部分内容阅读
目的:以离子液体1-丁基-3-甲基咪唑双三氟甲磺酰亚胺盐(BMIMNTF2)和羧甲基-β-环糊精(CM-β-CD)共同作为手性选择剂,建立分离美托洛尔和普萘洛尔混合对映体毛细管电泳方法。方法:采用未涂层毛细管(有效长度42cm,内径50μm),考察了离子液体的类型及浓度、缓冲溶液的浓度、缓冲溶液p H、分离电压对手性拆分的影响。结果:毛细管电泳同时分离美托洛尔对映体和普萘洛尔对映体的最佳电泳条件:以5mmol·L#1BMIMNTF2和5 mmol·L#1CM-β-CD共同作为手性选择剂,缓冲溶液为73 mmol·L#1pH 6.0的Tris-H3PO4溶液,分离电压为20 kV,检测波长为230 nm,高差10 cm进样15s,室温下进行。美托洛尔和普萘洛尔混合对映体在18 min内达到基线分离。结论:BMIMNTF2与CM-β-CD的协同作用不仅可以改善美托洛尔和普萘洛尔之间的分离,而且可以提高美托洛尔对映体和普萘洛尔对映体手性分离的选择性。
OBJECTIVE: To establish a chiral selector with ionic liquid 1-butyl-3-methylimidazolium bistrifluoromethanesulfonimide (BMIMNTF2) and carboxymethyl β-cyclodextrin (CM-β-CD) , A separation of metoprolol and propranolol mixed enantiomer capillary electrophoresis was established. Methods: The uncoated capillary (effective length 42cm, inner diameter 50μm) was used to investigate the effects of ionic liquid type and concentration, buffer concentration, buffer pH and separation voltage on chiral resolution. Results: The optimum electrophoresis conditions for simultaneous separation of enantiomers of metoprolol and propranolol by capillary electrophoresis were as follows: 5 mmol·L-1 # 1MIMNTF2 and 5 mmol·L # 1CM-β-CD were used as chiral selector , Buffer solution was 73 mmol·L # 1 pH 6.0 Tris-H3PO4 solution, the separation voltage was 20 kV, the detection wavelength was 230 nm, and the height difference of 10 cm was injected for 15 seconds at room temperature. The enantiomers of metoprolol and propranolol reached baseline separation within 18 min. CONCLUSION: The synergistic effect between BMIMNTF2 and CM-β-CD not only improves the separation between metoprolol and propranolol, but also enhances the chiral separation of metoprolol enantiomers and propranolol enantiomers The selectivity.