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目的:探讨补肾活血方、补肾方、活血方对血管性痴呆小鼠学习记忆能力及脑组织白介素1β(IL-1β)和肿瘤坏死因子α(TNF-α)含量的影响。方法:采用反复脑缺血再灌注法复制血管性痴呆小鼠模型,以尼莫地平为阳性对照组,观察补肾活血方、补肾方、活血方3组小鼠水迷宫法行为学及脑组织IL-1β和TNF-α含量的变化。结果:与假手术组比较,模型组小鼠学习与记忆能力下降(P<0.01),脑组织IL-1β和TNF-α含量明显升高(P<0.01);与模型组比较,各治疗组学习成绩和记忆成绩均有明显提高(P<0.05,P<0.01),脑组织IL-1β和TNF-α含量明显下降(P<0.05,P<0.01);各治疗组比较,补肾活血组的学习和记忆成绩优于其它治疗组(P<0.05),脑组织IL-1β和TNF-α含量低于其它治疗组(P<0.05,P<0.01)。结论:补肾活血系列方可通过降低脑组织IL-1β和TNF-α含量,改善血管性痴呆小鼠学习与记忆能力,且补肾活血方优于活血方和补肾方,活血方和补肾方无显著差异。
Objective: To investigate the effects of Bushen Huoxue Recipe, Bushen Recipe and Huoxue Recipe on the learning and memory abilities and the levels of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in brain tissues of mice with vascular dementia. Methods: The model of vascular dementia was duplicated by repeated cerebral ischemia and reperfusion. Nimodipine was used as the positive control group. The water maze behavior and the content of brain tissue IL were observed in rats of Bushen Huoxue Recipe, Bushen Recipe and Huoxue Recipe. -1β and TNF-α content changes. Results: Compared with the sham operation group, the learning and memory abilities of the model group decreased (P <0.01) and the levels of IL-1β and TNF-α in the model group increased significantly (P <0.01). Compared with the model group, (P <0.05, P <0.01), and the levels of IL-1β and TNF-α in brain tissues were significantly decreased (P <0.05, P <0.01). Compared with the other two groups, The scores of learning and memory were superior to those of other treatment groups (P <0.05). The levels of IL-1β and TNF-α in brain tissues were lower than other treatment groups (P <0.05, P <0.01). Conclusion: Bushen Huoxue Decoction can improve the learning and memory abilities of mice with vascular dementia by decreasing the levels of IL-1β and TNF-α in brain tissue. Bushen Huoxue Decoction is superior to Huoxue Decoction and Bushen Recipe. difference.