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用常规的石蜡制片法是不能制作含胶乳管的永久制片的,因为乳胶会在材料的脱水透明过程中被二甲苯或氯仿等溶剂所溶解。“碘-溴-冰醋酸变性法”,虽能获得良好的含胶乳管的永久制片,但用此法溴很易挥发,有毒,在没有良好通风设备的条件下操作,污染极为严重,有伤身体健康。我们不用溴,只用碘-冰醋酸进行变性处理,获得了比较满意的效果。现将制片操作过程介绍如下: 1.变性试剂的配制每100毫升冰醋酸中加4克碘,或在冰醋酸中加入过量的碘使成过饱和溶液,装入棕色试剂瓶中,存放一周后使用。此试剂的有效期可达一年以上,还可
Conventional paraffin preparation is not able to make permanent latex-containing tubes because the latex is dissolved in solvents such as xylene or chloroform during the dehydration of the material. “Iodine-bromine-acetic acid denaturation method”, although can get a good permanent latex sheet, but using this method is very volatile bromine, toxic, in the absence of a good ventilation equipment operation, the pollution is extremely serious, there Hurt health. We do not use bromine, only iodine - acetic acid denaturation, obtained more satisfactory results. Production process is now introduced as follows: 1. Preparation of denaturing reagents Add 4 g of iodine per 100 ml of glacial acetic acid, or excess iodine in glacial acetic acid to make a supersaturated solution into a brown reagent bottle for one week After use. This reagent is valid for more than one year, but also