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背景:消化性溃疡（PU）和十二指肠胃反流（DGR）患者的血浆血管活性肠肽（VIP）含量常高于正常水平,而幽门螺杆菌（H.pylori）感染可能参与PU的发病。目的:探讨PU患者的VIP与DGR和H.pylori感染的关系。方法:采用放射免疫测定（RIA）检测34例胃溃疡（GU）患者、42例十二指肠球部溃疡（DU）患者和30例健康人的血浆VIP含量;放射性核素99mTc-EHIDA显像法测定DGR;双抗体夹心酶联免疫吸附测定（ELISA）检测血清H.pylori IgG抗体,Giemsa染色检测胃黏膜H.pylori。结果:GU组的血浆VIP含量显著高于DU组和正常对照组（P<0.01）;DGR阳性率亦显著高于DU组（P<0.05）。DGR阳性组的血浆VIP含量显著高于DGR阴性组（P<0.01）。H.pylori阳性组的血浆VIP含量显著低于H.pylori阴性组（P<0.05）。结论:PU患者血浆VIP含量升高可能是DGR发生的重要因素之一。
BACKGROUND: The content of plasma vasoactive intestinal peptide (VIP) in patients with peptic ulcer (PU) and duodenal regurgitation (DGR) is often higher than normal, and H. pylori infection may be involved in the pathogenesis of PU . Objective: To investigate the relationship between VIP and DGR and H. pylori infection in PU patients. Methods: The plasma VIP levels of 34 patients with gastric ulcer (GU), 42 patients with duodenal ulcer (DU) and 30 healthy controls were detected by radioimmunoassay (RIA). The radionuclide 99m Tc-EHIDA imaging DGR was detected by enzyme-linked immunosorbent assay (ELISA), H. pylori IgG antibody was detected by ELISA and H.pylori was detected by Giemsa staining. Results: The plasma VIP level in GU group was significantly higher than that in DU group and normal control group (P <0.01). The positive rate of DGR in DU group was also significantly higher than that in DU group (P <0.05). Plasma VIP levels in DGR-positive group were significantly higher than those in DGR-negative group (P <0.01). The plasma VIP level of H.pylori positive group was significantly lower than that of H.pylori negative group (P <0.05). Conclusion: Elevated VIP in plasma of patients with PU may be one of the important factors of DGR.