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为了探讨生长因子自分泌刺激环路在喉癌发生发展中的作用。本研究采用逆转录聚合酶链式反应技术（RT－PCR），以β-actin为内参照，测定喉癌、癌旁正常喉粘膜及声带息肉组织转化生长因子-α（TGF-α）和表皮生长因子受体（EGFR）mRNA水平。结果显示，喉癌和癌旁组织TGF-α和EGFRmR－NA水平明显高于声带息肉组织（P＜0．05），而喉癌组织TGF－αmRNA水平又高于癌旁组织（P＜0．05）。表明TGF－α及其受体在喉癌的极早期，甚至尚未出现组织表型改变时，就可构成自分泌刺激环路，而且TGF－αmRNA表达水平的进一步上调在触发癌变中有重要作用。
In order to investigate the role of growth factor autocrine stimulation loop in the development of laryngeal carcinoma. In this study, RT-PCR andβ-actin were used as internal reference to detect the expression of transforming growth factor-α (TGF-α) and epidermal tissue in laryngeal carcinoma, normal laryngeal mucosa and vocal cord polyps Growth factor receptor (EGFR) mRNA levels. The results showed that the levels of TGF-α and EGFRmR-NA in laryngeal carcinoma and paracancerous tissues were significantly higher than those in vocal cord polyp (P <0.05), while the levels of TGF-αmRNA in laryngeal carcinoma were higher than that in paracancerous tissues (P <0.05). 05). It indicates that TGF-α and its receptor can form an autocrine stimulatory loop in the very early stage of laryngeal carcinoma even without the phenotypic change of TGF-αmRNA, and the further upregulation of TGF-αmRNA expression may play an important role in triggering carcinogenesis.