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目的:探讨一氧化氮供体S-亚硝基谷胱甘肽(GSNO)对储存期内机采血小板代谢的影响。方法:随机采集机采血小板6份,每份150~170ml,混匀后无菌分装于血小板专业保存袋中,共5袋,每袋30~35ml,4个试验组(向各组中每1袋血小板加入不同浓度GSNO溶液5ml,最终浓度依次为5、10、100和200μmol/L)和1个对照组(加入等量的无菌生理盐水),于(22±2)℃震荡保存7d,分别在第1、3、5、7天取血小板2ml进行血小板常规指标、血气分析、MTS等指标检测。结果:对照组NO浓度随时间增加而小幅增长,试验组中,高浓度组(100、200μmol/L)在第3天达到顶峰,低浓度组(5、10μmol/L)在第5天达到顶峰。血小板pH值在第7天保存期中呈下降趋势,且保存至第7天时仍符合血小板储存期末pH值要求。2组pH、MPV、PDW、pCO2、pO2、cHCO3等指标比较差异无统计学意义(P=0.07,P=0.47,P=0.59,P=0.15,P=0.64,P=0.06),随时间增长对照组和处理组pH、pCO2、cHCO3均逐渐降低趋势,而pO2L、MPV、PDW呈逐渐升高趋势。随GSNO浓度增大,Lac含量递减;随时间增长,Lac含量逐渐增加。保存期内血小板2组MTS活性随时间增长而递减,GSNO处理的血小板与对照组相比均差异无统计学意义(P=0.18,P=0.08,P=0.24,P=0.51)。结论:血小板保存过程中加入NO供体GSNO,一定程度上可以抑制血小板代谢,减少乳酸形成,改善血小板功能。
Objective: To investigate the effect of nitric oxide donor (S-nitrosoglutathione (GSNO) on the metabolism of organism platelets during storage). Methods: Randomly collected 6 platelets, 150 ~ 170ml each. After mixing, the cells were aseptically packed in a platelet-specific preservation bag with a total of 5 bags of 30 ~ 35ml per bag and 4 test groups One bag of platelets was added with 5 ml of different concentrations of GSNO solution, followed by 5, 10, 100 and 200 μmol / L) and 1 control group (with equal amount of sterile saline) and stored at (22 ± 2) , Respectively, in the 1st, 3rd, 5th and 7th days to take platelets 2ml conventional indicators, blood gas analysis, MTS and other indicators detection. RESULTS: In the control group, NO concentration increased slightly with time. In the experimental group, the high concentration group (100 and 200 μmol / L) peaked on the third day and the low concentration group (5 and 10 μmol / L) peaked on the fifth day . The platelet pH value showed a decreasing trend during the storage period of the 7th day and still meets the requirement of the end of the storage period of platelet by the 7th day. There was no significant difference in the indexes of pH, MPV, PDW, pCO2, pO2 and cHCO3 between the two groups (P = 0.07, P = 0.47, P = 0.59, P = 0.15, P = 0.64, P = 0.06) The pH, pCO2 and cHCO3 in the control group and the treatment group decreased gradually, while the levels of pO2L, MPV and PDW increased gradually. With the increase of GSNO concentration, the content of Lac decreased gradually; with the increase of time, Lac content increased gradually. During the storage period, the activity of MTS in platelet 2 decreased with time. There was no significant difference in the activity of GSNO between the platelets and the control group (P = 0.18, P = 0.08, P = 0.24, P = 0.51). Conclusion: NO donor GSNO during platelet preservation can inhibit platelet metabolism, reduce the formation of lactic acid and improve platelet function to a certain extent.