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目的研究肠道经中子照后的病变特点、bFGF蛋白和mRNA的表达及意义。方法采用2.5～5.5Gy中子照射230只BALBC小鼠,于照后6和12h、1～5d、7、10、14、21和28d分批活杀,采用免疫组化和原位杂交等技术研究bFGF基因在肠组织中的表达。结果照射后隐窝细胞见凋亡与坏死,并呈剂量相关性,2.5Gy组肠黏膜见明显损伤及恢复现象。bFGF蛋白和mRNA于正常肠上皮细胞阳性,其mRNA于血管内皮和间质细胞强阳性。2.5Gy照后3d内,bFGF蛋白进行性减少,照后5～10d,绒毛上皮细胞bFGF蛋白明显增加,5d达高峰,14d恢复至正常水平。4.0Gy以上照后4d内,bFGF进行性减少。bFGFmRNA与其蛋白出现相似的变化规律,其高峰见于照后3d,10d基本恢复至正常水平。结论一定剂量的中子辐射可使肠黏膜明显损伤,并呈剂量相关性;中子照射后肠内源性bFGF基因表达参与其损伤及修复的病理过程,可能对其损伤后的修复起促进作用。
Objective To study the pathological features of intestinal tract after neutron irradiation and the expression and significance of bFGF protein and mRNA. Methods 230 BALBC mice were irradiated with 2.5-5.5 Gy neutron and killed in groups at 6, 12, 1, 5, 7, 10, 14, 21 and 28 days after irradiation. Immunohistochemistry and in situ hybridization To study the bFGF gene expression in intestinal tissue. Results After irradiation, the crypt cells showed apoptosis and necrosis, and showed a dose-related relationship. The intestinal mucosa of 2.5Gy group showed obvious injury and recovery. bFGF protein and mRNA are positive in normal intestinal epithelial cells, and its mRNA is strongly positive in vascular endothelial and interstitial cells. The bFGF protein decreased progressively after 2.5Gy irradiation. The expression of bFGF protein in villus epithelial cells increased significantly from 5 to 10 days after irradiation, reaching the peak on the 5th day and returned to the normal level on the 14th day. Within 4 days after 4.0 Gy, bFGF progressively decreased. bFGFmRNA and its protein appeared similar changes, the peak seen in the photo after 3d, 10d returned to normal levels. Conclusions A certain dose of neutron radiation can significantly damage the intestinal mucosa and show a dose-dependent manner. The expression of endogenous bFGF gene after neutron irradiation is involved in the pathological process of injury and repair, which may play an important role in the repair after injury .