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该文探讨了长链非编码RNA(long non-coding RNA,1ncRNA)VLDLR在胰腺癌组织及5株细胞系中的表达,并分析了其对细胞增殖和迁移能力的影响。通过定量反转录PCR(quantitative reverse transcription PCR,qRT-PCR)检测VLDLR在14例胰腺癌组织和对应癌旁组织及5株细胞系中的表达水平,并进一步利用RNA干扰(ribonucleic acid interference,RNAi)技术探讨VLDLR的生物学功能。CCK-8和克隆形成实验检测细胞增殖能力,Transwell实验检测细胞迁移能力,Western blot检测蛋白质表达。结果发现,14例胰腺癌组织中,VLDLR相对表达水平为4.49±5.85(P<0.05),VLDLR在5株胰腺癌细胞中差异性表达。与对照组相比,实验组细胞中VLDLR m RNA水平明显降低(t分别为32.43、19.02,P<0.05);细胞增殖、细胞克隆形成及迁移能力明显均降低(P<0.05),基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)和MMP-9蛋白质水平降低(P<0.05)。该研究结果表明,VLDLR在胰腺癌中高表达,下调VLDLR表达可降低胰腺癌细胞的增殖和迁移能力。
This article explored the expression of long non-coding RNA (VnRNA) VLDLR in pancreatic cancer tissues and 5 cell lines, and analyzed its effect on cell proliferation and migration. The expression of VLDLR in 14 pancreatic cancer tissues and corresponding paracancerous tissues and 5 cell lines was detected by quantitative reverse transcription PCR (qRT-PCR), and the expression of VLDLR was further detected by using RNAi (RNAi ) Technology to explore the biological function of VLDLR. CCK-8 and colony formation assay were used to detect cell proliferation, Transwell assay was used to detect cell migration and Western blot was used to detect protein expression. The results showed that the relative expression level of VLDLR in 14 pancreatic cancer tissues was 4.49 ± 5.85 (P <0.05). VLDLR was differentially expressed in 5 pancreatic cancer cells. Compared with the control group, the levels of VLDLR m RNA in the experimental group were significantly decreased (t = 32.43, 19.02, respectively, P <0.05); the cell proliferation, cell colony formation and migration were significantly decreased -2 (matrix metalloproteinase-2, MMP-2) and MMP-9 protein levels decreased (P <0.05). The results show that VLDLR is highly expressed in pancreatic cancer, down-regulation of VLDLR expression can reduce the proliferation and migration of pancreatic cancer cells.