什么是λ基因组工程λ基因组工程(engineering of the ge-nome of the bacteriophage λ)就是在体内及体外定向改造λ DNA的一种生物技术。它是利用微生物遗传学和目前发展起来的高级细菌遗传学知识,生化手段将点突变,置换,插入,缺失等引入野生型及各种衍生型λDNA,或者将λDNA的某些片段引入其它来源的DNA(如质粒),以构成一个运载体使之携带外源DNA,并能转入敏感细胞得以扩增,以及再克隆与表达。可以说它是基因工程中的一项配套工程,随着这项工程的发展,由λDNA改造,或由λDNA片段参与 What is the lambda genome engineering? The genome engineering (engineering of the gemcnome of the bacteriophage lambda) is a biotechnique that directs the engineering of lambda DNA in vivo and in vitro. It is the use of microbial genetics and the current development of advanced bacterial genetic knowledge, biochemical means to point mutations, substitutions, insertions, deletions, etc. into wild-type and various derived lambda DNA, or some of the lambda DNA fragments into other sources DNA (such as plasmids) to form a carrier to carry foreign DNA, can be transferred to sensitive cells to be amplified, and then cloned and expressed. It can be said that it is a supporting project in genetic engineering. With the development of this project, it is modified by λDNA or by λDNA fragment