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目的：研究肾上腺素能受体激动剂与阻断剂对血管平滑肌细胞增殖、ＤＮＡ合成和ｃ－ｆｏｓ，ｃ－ｍｙｃ原癌基因表达的影响．方法：应用细胞培养、￣３Ｈ－胸腺嘧啶核苷掺入和斑点杂交的方法．结果：１～１０μｍｏｌ／Ｌ去甲肾上腺素（ＮＥ）和０．１～１０μｍｏｌ／Ｌ异丙基肾上腺素（ＩＳＯ）可明显促进离体培养的ＳＤ大鼠的主动脉平滑肌细胞增殖、￣３Ｈ－ＴｄＲ掺入和ｃ－ｆｏｓ，ｃ－ｍｙｃ原癌基因表达，并呈剂量依赖效应．该效应可为相应的受体阻断剂Ｐｈｅｎｔｏｌａｍｉｎｅ（１０μｍｏｌ／Ｌ）和Ｐｒｏｐｒａｎｏｌｏｌ（１０μｍｏｌ／Ｌ）所抑制（Ｐ＜０．０５）．结论：肾上腺素能受体激动剂可促进离体培养的大鼠主动脉平滑肌细胞的增殖、ＤＮＡ合成和原癌基因的表达，该效应可被其相应的阻断剂所抑制．
Objective: To investigate the effects of adrenergic receptor agonists and blockers on the proliferation of vascular smooth muscle cells, DNA synthesis and the expression of c-fos and c-myc oncogenes. Methods: Cell culture, ~ 3H-thymidine incorporation and dot blot hybridization method. RESULTS: 1 ~ 10μmol / L norepinephrine (NE) and 0.1 ~ 10μmol / L isoproterenol (ISO) significantly promoted the proliferation of aortic smooth muscle cells of SD rats cultured in vitro. TdR incorporation and c-fos, c-myc oncogene expression in a dose-dependent manner. This effect was inhibited by the corresponding receptor blockers Phentolamine (10μmol / L) and Propranolol (10μmol / L) (P <0.05). Conclusion: Adrenergic receptor agonist can promote the proliferation, DNA synthesis and proto-oncogene expression of rat aortic smooth muscle cells cultured in vitro. This effect can be inhibited by its corresponding blockers.