目的观察雷公藤内酯醇和华蟾素对乳腺癌细胞MCF-7增殖的影响。方法利用台盼蓝计数以及MTT法检测该药物对肿瘤细胞的抑制作用。结果雷公藤内酯醇在75~150 mg/L的剂量下可以对MCF-7细胞的生长产生明显的抑制作用;华蟾素在0.4 g/mL0、.2 g/mL的剂量下可对MCF-7细胞的生长产生明显的抑制作用;雷公藤内酯醇(75 mg/L)和华蟾素(0.2 g/mL)联合作用也可以对MCF-7细胞的生长产生明显的抑制作用;两者均有明显的时间依耐性。结论雷公藤内酯醇和华蟾素单独应用或者联合作用均可对乳腺癌细胞MCF-7的增殖产生抑制作用。 Objective To observe the effects of triptolide and cinobufacini on the proliferation of breast cancer cell line MCF-7. Methods The inhibitory effect of this drug on tumor cells was detected by trypan blue counting and MTT assay. Results Triptolide significantly inhibited the growth of MCF-7 cells at a dosage of 75-150 mg / L. Cinobufacine significantly inhibited the proliferation of MCF-7 cells at a dose of 0.4 g / mL0 and 0.2 g / mL, 7 cells. The combination of triptolide (75 mg / L) and cinobufacine (0.2 g / mL) could also significantly inhibit the growth of MCF-7 cells. Both of them There is obvious time according to patience. Conclusion Triptolide and cinobufacin alone or in combination can inhibit the proliferation of MCF-7 breast cancer cells.