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目的考察洗涤法结合硅珠法提取指甲垢DNA的应用价值。方法收集200枚刑事案件中受害人指甲,采用洗涤法结合硅珠法提取指甲垢DNA;用Identifiler试剂盒进行PCR扩增,3030xl遗传分析仪进行分型检测。结果应用洗涤法结合硅珠法在200枚指甲中有64枚(32%)检出异体DNA,其中52枚为混合型,12枚为单一异体DNA;另有104枚检出受害人单一DNA。结论洗涤法结合硅珠法可应用于指甲垢DNA的提取。
Objective To investigate the application value of washing nails and silica bead method to extract nails scale DNA. Methods The nails of 200 victims in criminal cases were collected. The nails DNA was extracted by washing method and silica bead method. The PCR products were amplified by using Identifiler kit and genotyped by 3030xl Genetic Analyzer. RESULTS: Allogeneic DNA was detected in 64 (32%) of the 200 nails by using the washing method combined with the silicon bead method. Among them, 52 were mixed and 12 were single allogeneic DNA. Another 104 detected single DNA of the victim. Conclusion Wash method combined with silica bead method can be applied to the extraction of nail polish DNA.