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目的 利用陡脉冲电场的可逆性电穿孔电转基因,观察陡脉冲治疗仪联合基因治疗对肿瘤生长的影响。方 法 构建抗H- ras基因表达的小发夹状RNA重组质粒载体(pshRNA H- ras);建立卵巢癌荷瘤裸鼠模型,光、电镜观察陡脉 冲对肿瘤组织的病理学改变,荧光报告质粒观察陡脉冲电场的可逆性电穿孔;实验分为4组:对照组、陡脉冲治疗组、陡脉 冲加基因单次干扰治疗组和加基因重复干扰治疗组,记录治疗后21d各荷瘤鼠肿瘤的体积和体质量。结果 陡脉冲对肿 瘤组织有明显的破坏作用,肿瘤边缘残存有活性的癌细胞;各治疗组肿瘤体积与体质量明显低于对照组,差异具有极显著 性(P<0.01),其中,陡脉冲加基因重复干扰治疗组肿瘤体积和重量显著低于仅用陡脉冲治疗组(P<0.01)。结论 陡脉 冲是一种新型有效的治癌工具,联合基因治疗能更有效地抑制肿瘤生长。
Objective To evaluate the effect of steep pulse therapy combined with gene therapy on tumor growth by reversible electroporation electroporation gene in steep pulse electric field. Methods A small hairpin RNA recombinant plasmid vector (pshRNA H-ras) expressing H-ras gene was constructed. A nude mouse model of ovarian cancer was established. The histopathological changes of the tumor tissue were observed under light and electron microscope. The fluorescence reporter plasmid The reversible electroporation of steep pulse electric field was observed. The experiment was divided into 4 groups: control group, steep pulse group, steep pulse plus gene single interference group and gene plus interference group. The volume and body mass. Results The steep pulse had a significant effect on the tumor tissue and there were remaining active cancer cells on the edge of the tumor. The tumor volume and body weight in each treatment group were significantly lower than those in the control group (P <0.01). Among them, steep pulse The tumor volume and weight in the plus gene interference group were significantly lower than those in the steep pulse alone group (P <0.01). Conclusion Steep pulse is a new and effective tool for cancer treatment. Combined gene therapy can inhibit tumor growth more effectively.