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目的探讨三七总皂苷(TSPN)对全脑缺血后成年大鼠侧脑室室管膜区(SVZ)神经再生的影响。方法采用四血管阻断法制作全脑缺血模型。大鼠分成假手术组、模型组和TSPN组。TSPN组大鼠全脑缺血后30 min ip给予剂量为75mg/kg的TSPN,每天1次,模型组给予等体积的生理盐水,连续14 d。分别于再灌注1、3、7、14 d处死大鼠,免疫组织化学染色观察SVZ区Brd U和微管相关蛋白Doublecortin(DCX)的表达,免疫荧光双标观察SVZ区Brd U/DCX、DCX/Ki67、GFAP/DCX的共表达情况。结果 TSPN组SVZ区7、14 d的Brd U+细胞数目均显著高于模型组对应的时间点(P<0.01、0.001);TSPN组和模型组SVZ区DCX+细胞的平均光密度值在7、14 d有统计学差异(P<0.01、0.001);TSPN组SVZ区14 d的Brd U/DCX细胞,均显著多于模型组(P<0.01);TSPN组SVZ区Ki67/DCX细胞在7、14 d的表达多于模型组,差异显著(P<0.01、0.001);两组SVZ区GFAP/DCX细胞与DCX的比值在3、7、14 d差异显著(P<0.05、0.001)。结论 TSPN促进全脑缺血后大鼠SVZ区神经再生,加速全脑缺血后大鼠SVZ区神经祖细胞增殖和分化,促进全脑缺血后大鼠SVZ区星形胶质细胞转化新生未成熟神经元。
Objective To investigate the effects of total saponins of Panax notoginseng (TSPN) on the neurogenesis in the lateral ventricular zone (SVZ) of adult rats after global cerebral ischemia. Methods The model of global cerebral ischemia was established by four-vessel occlusion method. Rats were divided into sham operation group, model group and TSPN group. The rats in TSPN group were given TSPN at a dose of 75 mg / kg ip 30 min after the onset of global cerebral ischemia. The rats in the model group were given equal volume of normal saline for 14 days. The rats were killed at 1, 3, 7 and 14 days after reperfusion. The expression of BrdU and microtubule-associated protein Doublecortin (DCX) in SVZ were observed by immunohistochemical staining. BrdU / DCX and DCX / Ki67, GFAP / DCX co-expression. Results The numbers of Brd U + cells in SVZ of TSPN group at 7 and 14 d were significantly higher than those in model group (P <0.01, 0.001). The average optical density of DCX + cells in TSPN group and SVZ group was 7, 14 (P <0.01, 0.001). The BrdU / DCX cells in the SVZ area of the TSPN group for 14 d were significantly more than that of the model group (P <0.01). In the TSPN group, the Ki67 / (P <0.01, 0.001). The ratio of GFAP / DCX cells to DCX in the two SVZ groups was significantly different at 3, 7 and 14 d (P <0.05, 0.001). Conclusion TSPN can promote nerve regeneration in SVZ of rats after global cerebral ischemia and accelerate the proliferation and differentiation of neural progenitor cells in SVZ of rats after global cerebral ischemia and promote the newborn of astrocyte transformation in SVZ of rats after global cerebral ischemia Mature neurons.