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应用ＬＣ－６Ａ高效液相色谱仪（ＨＰＬＣ）检测非感染与感染马来丝虫微丝蚴中华按蚊的血淋巴，均测出２０种游离氨基酸。经统计处理这些氨基酸的变化为：１．非感染中华按蚊的氨基酸总量于血餐后第５天比刚羽化后来吸血蚊增加３７％，以后一直维持在增加水平，但第１２天增加量稍减为２１９％。感染马来丝虫中华按蚊的氨基酸总量于血餐后第５天锐减２５．９％，第１２天又增加到２９．０％。２．两组蚊各种氨基酸变化多与其总量变化一致。而感染蚊与非感染蚊比较，则有１５种氨基酸含量具显著性差异（Ｐ＜０．０５），①谷氨酰胺、酪氨酸于血餐后１２ｄ前一直明显增加，甘氨酸＋苏氨酸、蛋氨酸于血餐后１２ｄ量显著增加；天门冬氨酸血餐后５ｄ量减少，１２ｄ也增加。②谷氨酸、丝氨酸、精氨酸、丙氨酸、缬氨酸、亮氨酸、赖氨酸血餐后１２ｄ前量均减少。③鸟氨酸血餐后５ｄ消失，１２ｄ又复出现，苯丙氯酸血餐后１２ｄ前一直未检出。
20 kinds of free amino acids were detected by LC-6A high performance liquid chromatography (HPLC) detection of hemolymph of Anopheles sinensis in non-infected and infected Malayan filarious microfilaria. The statistical treatment of these amino acid changes: 1. The total amount of amino acids in non-infected Anopheles sinensis increased 37% on the fifth day after the blood meal and later increased on the 12th day, while the increase was slightly reduced to 219% on the 12th day. The total amount of amino acids in infected Anopheles sinensis was 25.9% on the 5th day after blood meal, and increased to 29.0% on the 12th day. 2. Two groups of mosquitoes with more changes in the total amino acid changes consistent. However, compared with non-infected mosquitoes, there were 15 kinds of amino acids with significant difference (P <0.05). ① Glutamine and tyrosine increased significantly 12 days after blood meal, while glycine + threonine , Methionine significantly increased 12 days after blood meal; aspartate blood meal decreased 5d after meal, 12d also increased. ② glutamate, serine, arginine, alanine, valine, leucine, lysine before 12d after blood meal decreased. ③ ornithine blood meal disappeared 5d after the meal, 12d again appeared, 12 days before the meal of phenyl chloroform blood meal has not been detected.