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用聚合酶链反应技术对结核杆菌特异性重复序列ＩＳ６１１０部分基因１２３ｂＰ的片段进行扩增。该法检测人型结核杆菌灵敏度可达到１０ｆｇＤＮＡ，对１４９份结核患者临床标本检测结果显示ＰＣＲ总阳性率（６９．１％）明显高于直接涂片荧光镜检（１５．４％）与细菌培养（１０．１％）的阳性率（Ｐ＜０．０１）。研究表明ＰＣＲ是一种特异、敏感、快速诊断结核病的好方法，值得推广。
A fragment of 123bP of part of IS6110 gene of Mycobacterium tuberculosis was amplified by polymerase chain reaction (PCR). The sensitivity of this method for detection of Mycobacterium tuberculosis was 10fgDNA, and the positive rate of PCR in 149 samples of tuberculosis patients (69.1%) was significantly higher than that of direct smear fluoroscopy (15.4%) and bacterial culture (10.1%) (P <0.01). Studies have shown that PCR is a specific, sensitive, rapid method of diagnosis of tuberculosis is worth promoting.