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目的 :探讨牛磺酸对光照和暗适应条件下大鼠视网膜视杆细胞Gt1蛋白α亚基 (Gαt1)蛋白表达的影响。方法 :在幼年大鼠的饲料配方中添加牛磺酸 ,营养干预 4周后 ,采用免疫组织化学染色、Westernblot ting技术检测大鼠视网膜Gαt1在光照和暗适应条件下的分布和表达。结果 :光照时Gαt1主要表达于视杆细胞胞体及突触附近 ,暗适应时则以视杆外段的表达为主 ,且暗适应时Gαt1的蛋白表达量明显高于光照时。添加牛磺酸均可显著增加光照和暗适应时Gαt1蛋白的表达。结论 :Gαt1蛋白的分布及表达因光适应状态而异 ,牛磺酸能通过促进Gαt1表达 ,有利于视杆细胞光转导功能。
AIM: To investigate the effect of taurine on the expression of Gα1 protein in rat retinal retinal cells under light and dark adaptation. Methods: Taurine was added into juvenile rats’ diet. After 4 weeks of nutrition intervention, the distribution and expression of Gαt1 in retina under light and dark adaptation were detected by immunohistochemical staining and Western blotting. Results: Gαt1 was mainly expressed in the soma and synapse of rod-like cells when exposed to light. The expression of Gαt1 in dark adaptation was more than that in light. Addition of taurine significantly increased the expression of Gαt1 protein under light and dark adaptation. CONCLUSION: The distribution and expression of Gαt1 protein varies with light adaptation. Taurine can promote Gαt1 expression, which is in favor of photoreceptor function of rod photoreceptor cells.