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目的 探讨层黏连蛋白(LM)和Ⅳ型胶原在前列腺不同疾病中的表达及意义。方法 将100例诊断明确的前列腺标本分为5组,即正常组、良性增生组(BPH)、高级别上皮内瘤组(HPIN)、偶发癌组和前列腺癌组,每组20例。应用SP法检测LM和Ⅳ型胶原在5组标本中的表达。结果 LM和Ⅳ型胶原的表达基本一致,在正常组、BPH及大部分HPIN组织中呈棕黄色连续环线状包绕前列腺腺泡及导管外周,且分布均匀;但在部分HPIN组织中排列不规则、分布不均匀或紊乱;在偶发癌病灶区有片状缺失、间断或分布杂乱;在前列腺癌组织中均呈大片状或多灶性缺失,或杂乱排列。结论 LM和Ⅳ型胶原在前列腺不同疾病中的表达有差异,偶发癌和前列腺癌的基底膜损伤明显。提示LM和Ⅳ型胶原的表达不仅有助于预测HPIN的癌变潜能,也对前列腺良、恶性疾病的鉴别有帮助。
Objective To investigate the expression of laminin (Ⅳ) and type Ⅳ collagen in different diseases of prostate. Methods 100 cases of well-differentiated prostate specimens were divided into 5 groups: normal group, benign hyperplasia group (BPH), high-grade intraepithelial neoplasia group (HPIN), incidental cancer group and prostate cancer group, 20 cases in each group. The expression of LM and type Ⅳ collagen in 5 groups of specimens was detected by SP method. Results The expressions of LM and type Ⅳ collagen were basically the same. In the normal group, BPH and most of the HPIN tissues, the brown and yellow continuous wrapping around the prostatic acini and the periphery of the catheter, with uniform distribution. However, some HPIN tissues were irregularly arranged , Uneven distribution or disorder; in occasional lesions of the area flaky, interrupted or distributed disorder; in prostate cancer were large or multi-focal lesions were missing, or cluttered. Conclusions The expressions of LM and type Ⅳ collagen in different diseases of prostate are different. The damage of basement membrane of incident and prostate cancer is obvious. Tip LM and type Ⅳ collagen expression not only help predict the cancer potential of HPIN, but also for the identification of benign and malignant diseases of the prostate helpful.