论文部分内容阅读
目的探讨肥胖对非致死性肺炎小鼠局部组织炎症反应的影响。方法将高脂诱导的肥胖小鼠分为I、II组,常规非肥胖小鼠分为III、IV组,I、III组滴鼻40μl含4×109CFU大肠杆菌的菌液,II、IV组滴鼻40μl生理盐水,于感染2、6、12、24、48、72、96 h检测各组小鼠肺脏细胞因子、炎性细胞数量和组织学变化。结果与IV组比较,II组肺泡灌洗液中WBC、GRA、LYM、MID数量显著升高(P<0.05或P<0.01),支气管及肺泡壁周围炎性细胞浸润增加,肺匀浆中TNF-α、IL-8、IL-12、MIP-2质量浓度极显著升高(P<0.01);感染后,I、III组肺泡灌洗液中WBC和肺匀浆中TNF-α、IL-8、IL-12、MIP-2质量浓度先升高后降低,在96 h时降至对照组水平,支气管及肺泡隔细胞浸润、炎性渗出逐渐增加,肺脏炎性损伤逐渐加重,后在96 h时见消散;与III组比较,I组在感染第2~72 h期间,肺泡灌洗液中WBC数量、细胞因子质量浓度显著升高(P<0.05或P<0.01),炎性浸润更多、范围更广,96 h时细胞因子质量浓度明显降低、肺泡结构恢复显著。结论感染非致死肺炎后,肥胖能诱导更多炎性细胞和细胞因子在肺脏表达,有利于后期局部组织结构与功能的恢复。
Objective To investigate the effect of obesity on local tissue inflammatory response in non-lethal pneumonia mice. Methods Fat-induced obese mice were divided into groups I and II. Conventional non-obese mice were divided into groups III and IV. Groups I and III were intranasally injected with 40 μl of bacterial liquid containing 4 × 109 CFU of E. coli. Groups II and IV Nasal 40μl of saline, in the infection of 2,6,12,24,48,72,96 h lung tissue were detected in each group of cytokines, inflammatory cells and histological changes. Results Compared with group IV, the numbers of WBC, GRA, LYM and MID in group II were significantly increased (P <0.05 or P <0.01), the infiltration of inflammatory cells in bronchus and alveolar wall increased, The levels of TNF-α, IL-8, IL-12 and MIP-2 in bronchoalveolar lavage fluid in groups I and III were significantly higher than those in control group (P <0.01) 8, IL-12 and MIP-2 firstly increased and then decreased, and then decreased to the level of control group at 96 h. The infiltration and exudation of bronchial and alveolar septum gradually increased, the lung inflammatory injury gradually increased, (P <0.05 or P <0.01). Compared with group III, the number of WBC and the concentration of cytokines in alveolar lavage fluid in group I were significantly increased (P <0.05 or P <0.01) More, a broader range, 96 h cytokines mass concentration was significantly reduced, alveolar structural recovery significantly. Conclusion After infection with non-lethal pneumonia, obesity can induce more inflammatory cells and cytokines expressed in the lungs, which is conducive to the recovery of local tissue structure and function.