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目的 :寻找电刺激肋间神经调节胃功能活动的化学神经基础。材料和方法 :用荧光素双标记结合还原型尼克酰胺腺苷二核苷酸磷酸 -黄递酶 ( nicotin amide adenine dinucleotide phosphate- diaphorase,NADPH- d)组化方法和降钙素基因相关肽 ( calcitonin gene- related peptide,CGRP)免疫组化方法 ,观察了大鼠周围突向肋间神经和胃分支投射的脊神经节细胞是否含一氧化氮 ( nitric oxide,NO)或 NO和 CGRP。结果 :分别有 2 1.6%和 11.8%的周围突向肋间神经和胃分支投射的脊神经节细胞呈 NADPH- d阳性和 NADPH- d/ CGRP阳性 ,4 7.9%的胃初级传入脊神经节细胞呈 NADPH- d/ CGRP阳性。结论 :大鼠周围突向躯体和内脏分支投射脊神经节细胞内含 NO或 NO和CGRP共存 ,NO和 CGRP还共存于内脏初级传入脊神经节细胞。
OBJECTIVE: To find out the chemical neural basis for electrical stimulation of intercostal nerves regulating gastric function. MATERIALS AND METHODS: Combined with fluorescein double labeling and nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemistry and calcitonin gene-related peptide (CGRP) immunohistochemical method was used to observe whether the ganglion cells projecting from the intercostal and intercostal processes projecting to the stomach of the rats contain nitric oxide (NO) or NO and CGRP. RESULTS: Spinal ganglion cells projecting from the intercostal nerve and gastric branches projecting at 21.6% and 11.8% respectively were positive for NADPH-d and positive for NADPH-d / CGRP. Primary passage into the spinal ganglion was found in 4 7.9% of the patients NADPH-d / CGRP positive. CONCLUSIONS: Projections of NO or NO and CGRP in the ganglion cells projected by the somatic and visceral branches around the rat coexist. NO and CGRP also coexist in primary afferent spinal ganglion cells.