【摘 要】
：
AIM: To observe the effect of β-ionone on the proliferation of human gastric adenocarcinoma cell line SGC-7901 and the inhibition of metalloproteinase.METHODS:
【机 构】
：
Public Health College, Harbin Medical University, Harbin 150001,Heilongjiang Province, China“,”Depar
论文部分内容阅读
AIM: To observe the effect of β-ionone on the proliferation of human gastric adenocarcinoma cell line SGC-7901 and the inhibition of metalloproteinase.METHODS: Using growth inhibition, Zymograms assays and reverse transcription-polymerase-chain reaction(RT-PCR), we examined cell growth rates, activities of matrix metalloproteinases-2 (MMP-2) and -9 (MMP-9), and expression of metalloproteinases-1 (TIMP-1) and -2 (TIMP-2) in SGC-7901 cells after the treatment with β-ionone for 24h and 48 h, respectively.RESULTS: β-ionone had an inhibitory effect on the growth of SGC-7901 cells. Eight days after the treatment with β-ionone at concentrations of 25, 50, 100 and 200 μmol/L,the inhibition rates were 25.9%, 28.2%, 74.4% and 90.1%,respectively. The IC50 value of β-ionone for SGC-7901 cells was estimated to be 89 μmol/L. The effects of β-ionone on MMP-2 and MMP-9 activities in SGC-7901 cells were not observed. However, the levels of TIMP-1 and TIMP-2 transcripts were elevated in cells treated with β-ionone in a dose-dependent manner.CONCLUSION: β-ionone can inhibit the proliferation of SGC-7901 cells, upregulate the expression of TIMP-1 and TIMP-2 expression, and may influence metastasis of cancer.
其他文献
目的 分析在早期糖尿病肾病患者治疗中应用他汀类药物治疗的临床效果.方法 两组患者均采取常规治疗方案,观察组患者则在该基础上应用阿托伐他汀钙治疗.结果 两组患者治疗前血
目的 探讨血清癌抗原125(CA125)、癌抗原19-9(CA19-9)和癌胚抗原(CEA)水平在大肠癌诊断中的应用价值.方法 选择78例大肠癌手术患者为观察组,39例健康体检者为对照组,检测两组血清CA125、CA19-9、CEA水平.结果 观察组血清CA125、CA19-9、CEA水平分别为(33.67±50.42) μg/L、(46.67±26.08) kU/L和(17.52±24.17)
目的:分析红臀预警报告表在小儿腹泻护理中的应用效果.方法:回顾性分析我院2017年6月-2018年6月收治的82例小儿腹泻患儿资料,随机将其分为研究组和对照组,各41例,对照组实施
【摘要】 目的 探讨慢性肾功能衰竭血液透析患者营养状况进行评价和分析。方法 回顾本科2006年1月至2008年12月持续血液透析158例患者进行营养状况评价和分析。结果 158例持续血液透析患者进行评价,发生营养不良患者97例,发生率61.39%。其中轻 中度营养不良81例,发生率51.26%;重度营养不良16例,发生率10.12%。结论 认识到导致营养不良的临床因素,在护理方面加强,心理教育,正
AIM: To study the effect of staurosporine (ST) on the cell cycle of human gastriccancer cell lines MGC803 and SGC7901.METHODS: Cell proliferation was evaluated
目的 观察白三烯受体拮抗药孟鲁司特钠对致敏小鼠气道高阻力及α-平滑肌肌动蛋白(α-SMA)和转化生长因子-β1( TGF-β1)蛋白表达的影响.方法 将BALB/c小鼠随机分为正常组、模
目的 探讨经尿道等离子体前列腺腔内剜除术(PKERP)的技巧改进与应用.方法 应用Gyrus等离子内镜系统,对65例前列腺增生症患者行PKERP.整个手术技巧的掌握分3个步骤:前列腺尖部撬剥(10例),分叶撬剥(20例),腔内剜除(35例).结果 65例患者均手术成功,手术时间(78±24) min,切除腺体组织质量(69.0±9.4)g,术中出血量(110±35) ml.术后有2例拔除导尿管后排
目的 研究马兜铃酸(AA)作用下大鼠肾小管上皮细胞尾加压素II(UII)含量的变化.方法 采用机械分离和酶消化法获取大鼠肾小管上皮细胞并进行培养、鉴定;用RT-PCR和Western blot