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以甜叶菊茎段为外植体,采用不同低浓度秋水仙素在甜叶菊试管苗茎段再生苗初期进行长时间持续处理,以探讨低浓度秋水仙素诱导甜叶菊多倍体的优势及适合长时间诱导甜叶菊的秋水仙素浓度,简化甜叶菊离体诱导多倍体技术环节。结果表明:0.025%的秋水仙素浓度适合于甜叶菊多倍体离体诱导的持续诱变,直至试管苗再生而无需清洗外植体和更换培养基,简化了诱导技术环节,提高了甜叶菊多倍体离体诱导效率;多倍体植株田间农艺性状表现为叶片大而厚实、开花延迟、花蕾较大等特征。旨在建立低浓度秋水仙素离体诱导甜叶菊多倍体技术体系。
Stevia stem segments as explants, using different concentrations of colchicine in the early stage of regeneration of Stevia rebaudiana seedlings stems for a long time to deal with low concentrations of colchicine-induced stevia polyploid advantage and suitability For a long time, the concentration of colchicine in Stevia was induced to simplify the in vitro induced polyploidy of Stevia. The results showed that the colchicine concentration of 0.025% was suitable for the continuous mutagenesis induced by in vitro induction of Stevia rebaudiana until the in vitro plantlets were regenerated without cleaning the explants and changing the medium, which simplified the induction technology and increased the content of stevia Polyploid in vitro induction efficiency; polyploid field performance of agricultural plants as large and thick leaves, flowering delay, larger buds and other characteristics. Aim To establish a low concentration of colchicine in vitro induction of stevia polyploid system.