在进行心血管疾病的形态学实验研究中,常需借助心肌胶原的特殊染色。虽现有很多先进的组化染色可显示心肌胶原,但传统的Ven Gieson氏苦味酸酸性复红染色仍不失为优良方法,其色调艳丽,对两种纤维具有选择性着色能力,红黄对比鲜明,便于观察及图像分析。该染色操作虽然简单,但往往染色结果并不满意。笔者经过反复尝试后发现,只有把握好以下3个关键点方可得到理想的染色结果。 Morphological experiments in the study of cardiovascular disease, often require the special staining of myocardial collagen. Although many advanced histochemical stains can display myocardial collagen, the traditional Ven Gieson’s picric acid complex complex red dyeing is still an excellent method, its hue is bright, has selective coloring ability for both fibers, contrasting red and yellow, Easy to observe and image analysis. Although the dyeing operation is simple, the dyeing result is not satisfactory. After repeated attempts, I found that only by grasping the following three key points can be the ideal staining results.