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本文用两组辐照饲料对Wistar大鼠进行了110天和150天喂养,观察其骨髓多色性红细胞(PCE)的微核效应。一组为混合辐照饲料组,辐照饲料占80％;另一组为辐照蔬菜组,辐照蔬菜占总饲料的3％,辐照剂量为2KGy,是实际需要的辐照剂量2倍以上。同时设有普通饲料的阴性对照组,和用环磷酰胺处理或给以全身整体照射的阳性对照组。微核测定采用Giemsa染色法、Harris苏本精复染法和荧光染色法,两批实验均表明,在本实验条件下,不论是喂养混合辐照饲料还是辐照蔬菜,都未见到大鼠骨髓PCE微核细胞率有所增高。
In this study, Wistar rats were fed with two groups of irradiated diet for 110 days and 150 days, and the micronucleus effect of bone marrow erythrocytic (PCE) cells was observed. One group is a mixed radiation group, and the irradiated group accounts for 80%. The other group is a radiation vegetable group. Irradiated vegetables account for 3% of the total. The radiation dose is 2KGy, which is 2 times the actual radiation dose. the above. At the same time, there were a negative control group of common feed, and a positive control group treated with cyclophosphamide or given whole body irradiation. The micronucleus assay was performed using Giemsa staining, Harris Suppli, and fluorescent staining. Both batches of experiments showed that under the experimental conditions, no rats were fed either with mixed irradiated diet or irradiated vegetables. The rate of PCE micronucleated cells in bone marrow increased.