目的：建立血浆中普罗帕酮对映体浓度的立体选择性高效液相色谱测定法。方法：血浆标本经碱化以正己烷异戊醇（９９∶１）提取、吹干后与ＧＩＴＣ柱前衍生化，形成非对映体。以乙晴水冰醋酸（６０∶４０∶０．０１）为流动相，经Ｃ１８ＯＤＳ柱分离后在紫外λ２０８ｎｍ处检测。结果：对映体曲线范围２５μｇ·Ｌ－１～８００μｇ·Ｌ－１；最低检出限＜２０μｇ·Ｌ－１；批内、批间平均ＲＳＤ均＜６％；平均回收率：１０２．２％；临床大多数常用药物对本法无干扰。结论：本法为普罗帕酮临床药动药效学研究提供了一种简便、可行的检测方法 Objective: To establish a stereoselective HPLC method for the determination of enalapril enantiomers in plasma. Methods: The plasma samples were alkalized by n-hexane-isoamyl alcohol (99: 1), dried, and derivatized with GITC column to form diastereomers. To B Qing  water  glacial acetic acid (60:40:0.01) as the mobile phase after separation by C18 ODS column at UV λ208nm at the detection. Results: The enantiomer curve ranged from 25μg · L-1 to 800μg · L-1; the lowest detection limit was <20μg · L-1; the average RSD within and between batches was <6%; the average recovery was 102.2% Most clinically used drugs have no interference with this law. Conclusion: This method provides a simple and feasible method for the study of pharmacokinetics-pharmacodynamics of propafenone