侵袭和转移是恶性肿瘤最显著的特征,Matrigel穿膜侵袭实验是研究肿瘤细胞在体外侵袭能力的常用实验之-,经常使用Boyden小室体外侵袭实验检测肿瘤细胞的侵袭潜能。但是在实验中常常并不能获得理想的结果,其原因是没有掌握实验的具体操作细节。我们经过反复的实践,通过技术改进,? Invasion and metastasis are the most prominent features of malignant tumors. Matrigel transmembrane invasion assay is a commonly used experiment to study the invasive ability of tumor cells in vitro. Boyden chamber in vitro invasion assay is often used to detect the invasive potential of tumor cells. However, ideal results are often not obtained in experiments because the specific details of the experiments are not mastered. After repeated practice, we have adopted technological improvements.