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目的:建立HPLC测定龙须藤中5,7,3’,4’,5’-五甲氧基黄酮和5,6,7,5’-四甲氧基-3’,4’-亚甲二氧基含量的方法。方法:DIKMA PlatisilODS C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈(A)-0.1%磷酸溶液(B),梯度洗脱(0 min,25%A;15 min,45%A;40 min,50%A),流速0.8 mL·min-1,检测波长324 nm。结果:5,7,3’,4’,5’-五甲氧基黄酮和5,6,7,5’-四甲氧基-3’,4’-亚甲二氧基分别在0.008 4~0.336 0μg(r=0.999 9),0.013 4~0.536 0μg(r=0.999 7)与峰面积呈良好的线性关系,平均回收率分别为99.41%,98.65%,RSD分别为0.57%,1.01%。结论:该方法操作简单准确,分离效果、精密度和重复性好,适合于测定该药材中5,7,3’,4’,5’-五甲氧基黄酮和5,6,7,5’-四甲氧基-3’,4’-亚甲二氧基的含量。
Objective: To establish a HPLC method for the determination of 5,7,3 ’, 4’, 5’-pentamethoxyflavone and 5,6,7,5’-tetramethoxy-3 ’, 4’- Dioxy content of the method. METHODS: DIKMA Platisil ODS C18 column (4.6 mm × 250 mm, 5 μm), mobile phase acetonitrile (A) -0.1% phosphoric acid solution (B), gradient elution (0 min, 25% A; 15 min, 45% A; 40 min, 50% A), the flow rate was 0.8 mL · min-1 and the detection wavelength was 324 nm. Results: 5,7,3 ’, 4’, 5’-Pentamethoxyflavone and 5,6,7,5’-tetramethoxy-3 ’, 4’-methylenedioxy were respectively 0.008 4 ~ 0.336 0 μg (r = 0.999 9) and 0.013 4 ~ 0.536 0 μg (r = 0.999 7), respectively. The average recoveries were 99.41% and 98.65%, respectively, with RSDs of 0.57% and 1.01%, respectively. Conclusion: The method is simple, accurate, accurate and reproducible. It is suitable for the determination of 5,7,3 ’, 4’, 5’-pentamethoxyflavone and 5,6,7,5 ’- tetramethoxy-3’, 4’-methylenedioxy content.