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目的探讨苏尼替尼(Sutent)对HeLa细胞增殖及3-磷酸甘油醛脱氢酶(Glyceraldehyde-3-phosphate dehydro-genase,GAPDH)表达和活性的影响。方法用不同浓度的Sutent作用于HeLa细胞,采用MTT法检测Sutent对HeLa细胞增殖活力的影响;Western blot检测HeLa细胞中GAPDH蛋白表达的变化;GENMED细胞GAPDH活性终点比色法定量检测了GAPDH的活力。结果经Sutent处理24 h后,HeLa细胞的增殖受到明显抑制,且呈浓度依赖性,半数抑制浓度(IC50)为10.283μmol/L;Sutent可明显降低HeLa细胞中GAPDH蛋白的表达量,并能直接抑制GAPDH的活力。结论 Sutent可抑制HeLa细胞的增殖,降低GAPDH蛋白的表达量及活力,显示了其作为多靶点药物的重要研究价值。
Objective To investigate the effects of Sutent on the proliferation and the expression of GAPDH and the activity of HepG2 cells in HeLa cells. Methods The effect of Sutent on proliferation of HeLa cells was detected by MTT assay. The changes of GAPDH protein expression in HeLa cells were detected by Western blot. The activity of GAPDH was determined by end-point colorimetric assay of GAPDH activity in GENMED cells. . Results After Sutent treatment for 24 h, the proliferation of HeLa cells was significantly inhibited in a concentration-dependent manner with IC50 of 10.283 μmol / L. Sutent significantly decreased the expression of GAPDH protein in HeLa cells, Inhibit the vitality of GAPDH. Conclusion Sutent can inhibit the proliferation of HeLa cells and decrease the expression and viability of GAPDH protein, which shows its important value as a multi-target drug.