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本文报道根际区他感化学物质对小麦、玉米等作物种子萌发及幼苗生长抑制作用的研究结果。土壤及土壤溶液样品提取物,用GC及GC-MS鉴定出各类化合物50多种。重点研究有代表性的3种化合物:十四烯-1、苯甲醛以及未见报道的,由作者鉴定出的他感化合物:对-叔丁基苯甲酸。结果表明,十四烯-1在浓度达2000mg/l时,对玉米、小麦、田菁、稗子的种子萌发,幼根及幼芽生长均无明显影响;苯甲醛浓度在200mg/l以上时表现出对试验种子萌发及幼苗生长有抑制作用;对-叔丁基苯申酸在100mg/l时就对试验种子有显著抑制作用,并且随浓度增加抑制效果越强烈,在500mg/l时,小麦,玉米的萌发率,分别为对照的41%及7%,根生长分别为对照的5.6%及3.5%。并且,它严重抑制了幼苗生长。对-叔丁基苯甲酸和苯甲醛对试验作物种子用t0.01检验,表明当浓度大于100mg/l时与对照比,差异很显著。此外,他感化合物在小于50mg/l低浓度时,对作物种子有一定刺激作用。
This article reports on the rhizosphere area of his chemical substances on wheat, corn and other crops seed germination and seedling growth inhibition results. Soil and soil solution sample extracts, GC and GC-MS identified more than 50 kinds of compounds. Focused on three typical compounds: tetradecene-1, benzaldehyde and his previously identified compound, p-tert-butylbenzoic acid, which has not been reported. The results showed that when the concentration of tetradecene was 2000mg / l, there was no significant effect on the seed germination, young root and sprout growth of corn, wheat, Sesbania, and barnyardgrass. When the concentration of benzaldehyde was above 200mg / l Out of the experimental seed germination and seedling growth inhibition; p-tert-butyl benzene acid at 100mg / l had significant inhibitory effect on the test seed, and inhibit the effect with increasing concentration the more intense, at 500mg / l, wheat , The germination rate of corn were 41% and 7% of the control respectively, and the root growth was 5.6% and 3.5% respectively of the control. And, it severely inhibits seedling growth. P-tert-Butylbenzoic acid and benzaldehyde on the test crop seeds t0.01 test showed that when the concentration is greater than 100mg / l compared with the control, the difference is significant. In addition, he felt the compounds at a concentration of less than 50mg / l, the crop seeds have a certain stimulating effect.