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目的:使用乳液法制备含有神经生长因子(NGF)的电纺纤维,研究其外观形貌和机械强度等物理性能,以及制备过程中NGF活性的变化,纤维中NGF的担载量和纤维体外释放动力学,评价其能否成为理想的神经修复材料,为进一步将NGF电纺纤维应用于周围神经修复奠定基础。方法:将NGF水溶液分散于PLLA溶液,通过W/O乳液法制备静电纺丝缓释纤维,对纤维的外观形貌等物理性能等进行表征,使用Elisa方法测定制备过程中NGF活性的保持以及体外释放动力学。结果:NGF电纺纤维具备类似细胞外基质(ECM)的良好外观形貌和一定的机械强度,其中NGF活性保持19.58%±6.05%,体外有效释放11天。结论:本文制备的乳液法NGF电纺纤维具备良好的物理性能,能够持续缓释有效剂量的NGF,适合作为神经修复材料进行进一步研究。
OBJECTIVE: To prepare electrospun fibers containing nerve growth factor (NGF) by emulsion method and to study the physical properties such as appearance and mechanical strength, as well as the changes of NGF activity during preparation, the amount of NGF loaded in the fibers and the in vitro release of fibers Kinetics and evaluate whether it can be an ideal nerve repair material, which laid the foundation for further application of NGF electrospun fibers in peripheral nerve repair. Methods: The aqueous solution of NGF was dispersed in PLLA solution, and electrospun and sustained-release fibers were prepared by W / O emulsion method. The physical and chemical properties of the fibers were characterized. The Elisa method was used to determine the activity of NGF during the preparation and the in vitro Release kinetics. Results: The NGF electrospun fibers possessed similar appearances and mechanical strength similar to those of extracellular matrix (ECM). The NGF activity was maintained at 19.58% ± 6.05% and released effectively in vitro for 11 days. CONCLUSION: The NGF electrospun fibers prepared in this paper possess good physical properties and sustained sustained release of effective dose of NGF, which is suitable for further study as a nerve repair material.