论文部分内容阅读
对产卵前的雌鳗注射了不同剂量的甲状腺素(T4),然后利用放射性免疫法(RIA)测定了雌鳗血清、卵、胚胎及幼苗各发育时间段三碘甲腺原氨酸(T3)和甲状腺素(T4)的含量变化。结果发现,注射外源T424h后,随着雌鳗血清中的T4含量达到峰值,T3含量也达到了峰值。说明日本鳗鲡体内存在5’-单脱碘酶。与此同时,随着血清中T3和T4含量达到峰值,卵中的T3和T4含量也达到了峰值,进而证明母体中的T3和T4能够转入鳗鲡幼苗。进一步调整T4的注射剂量和时间后发现,在21℃水温条件下,T4的剂量为10～15mg·(500g)-1时,注射催产针前12h注射的效果最好。用该方法注射T4后,幼苗各发育时间段的存活率与对照组比较存在显著差异,出膜第9天的存活率由对照组的1.3%提高到22.0%。但日本鳗鲡幼苗最终未能存活,说明甲状腺素并不是影响日本鳗鲡幼苗存活的唯一因素。
Different doses of thyroxine (T4) were injected into females before spawning, and the contents of T3, T3, T3 and T3 in the developmental stages of serum, eggs, embryos and seedlings of female eel were determined by radioimmunoassay (RIA) ) And thyroxine (T4) content changes. The results showed that after the T424h injection, the T3 content peaked as the T4 content in the serum of the female eel peaked. It shows that there is 5’- In the meantime, as T3 and T4 levels peaked in serum, the T3 and T4 levels in the eggs peaked, further demonstrating that maternal T3 and T4 could be transferred into eel seedlings. Further adjustment T4 injection dose and time found that the 21 ℃ water temperature conditions, T4 dose of 10 ~ 15mg · (500g) -1, injection of oxytocin 12h injection best. After the injection of T4 by this method, the survival rate of seedlings at different developmental stages was significantly different from that of the control group. The survival rate on the 9th day after the treatment increased from 1.3% to 22.0% in the control group. However, the Japanese eel seedlings eventually failed to survive, indicating that thyroid hormone is not the only factor affecting the survival of Japanese eel seedlings.