论文部分内容阅读
目的星形胶质细胞瘤上调基因1(Astrocyte elevated gene-1,AEG-1)具有调控细胞转化、抑制凋亡和促进侵袭转移等多种生物学功能,其编码的蛋白质又叫异黏蛋白(Metadherin,MTDH),但是国内外研究者对AEG-1/MTDH与结肠癌及其病理特征和预后关系尚存争议,本研究应用Meta分析方法研究AEG-1/MTDH在人结直肠癌组织中表达的临床意义。方法检索CNKI、维普、万方和PubMed数据库,收集2001-01-2015-07的国内外专业期刊公开发表的文献、学位论文及学术会议的相关文献,根据结直肠癌标本的临床分期、病理分级、淋巴结转移和预后以及AEG-1/MTDH蛋白的检测和评价结果,采用NOS量表作为纳入标准筛选文献。应用RevMan 5.3软件对各研究原始数据进行统计处理,计算合并OR、RR及95%CI,同时绘制Meta分析森林图和漏斗图。并应用StataSE 12.0软件进行敏感性分析。结果共有11篇文献纳入系统评价,对原始数据AEG-1/MTDH蛋白阳性表达率进行Meta分析,结果显示,结直肠癌组AEG-1/MTDH表达高于正常对照组,OR=13.55,95%CI为6.06~30.30,P<0.01;Ⅲ+Ⅳ期低于Ⅰ+Ⅱ期,OR=3.66,95%CI为2.55~5.24,P<0.01;高、中分化组低于低分化组,OR=1.44,95%CI为1.11~1.85,P<0.01;淋巴结转移组高于无淋巴结转移组,OR=3.32,95%CI为2.44~4.52,P<0.01;AEG-1/MTDH为结直肠癌患者独立预后因素,RR=1.57,95%CI为1.08~2.29,P=0.02。结论 AEG-1/MTDH蛋白表达升高可能在结直肠癌的发生和发展中起重要作用,检测AEG-1/MTDH蛋白对指导结直肠的临床诊治有重要参考意义。
Objective Astrocyte elevated gene-1 (AEG-1) has many biological functions such as regulating cell transformation, inhibiting apoptosis and promoting invasion and metastasis. The protein encoded by it is also called as isoclone Metadherin, MTDH), but domestic and foreign researchers on the relationship between AEG-1 / MTDH and colon cancer and its pathological features and prognosis is still controversial, the study Meta analysis of AEG-1 / MTDH expression in human colorectal cancer tissue The clinical significance. Methods The databases of CNKI, VIP, Wanfang and PubMed were searched, and the relevant literatures, dissertations and academic conferences published in domestic and overseas journals from January 2001 to January 2015 were collected. According to the clinical stage, pathological grade of colorectal cancer , Lymph node metastasis and prognosis and AEG-1 / MTDH protein detection and evaluation results, the use of NOS scale included as a standard screening literature. The RevMan 5.3 software was used to perform statistical analysis on the original data of each study. The combined OR, RR and 95% CI were calculated and the Meta-analysis forest diagram and funnel chart were drawn. The sensitivity analysis was performed using StataSE 12.0 software. Results A total of 11 articles were included in the systematic review. Meta-analysis of the positive rates of AEG-1 / MTDH protein in the primary data showed that the expression of AEG-1 / MTDH in colorectal cancer group was higher than that in the normal control group (OR = 13.55,95% CI = 6.06-30.30, P <0.01; stage Ⅲ + Ⅳ was lower than stage Ⅰ + Ⅱ, OR = 3.66, 95% CI 2.55-5.24, P <0.01; 1.44, 95% CI 1.11-1.85, P <0.01; lymph node metastasis was higher than that without lymph node metastasis (OR = 3.32, 95% CI 2.44 ~ 4.52, P <0.01); AEG-1 / MTDH was significantly higher in patients with colorectal cancer Independent prognostic factors, RR = 1.57, 95% CI 1.08 ~ 2.29, P = 0.02. Conclusion The increased expression of AEG-1 / MTDH protein may play an important role in the pathogenesis and progression of colorectal cancer. Detection of AEG-1 / MTDH protein has important reference value for clinical diagnosis and treatment of colorectal cancer.