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目的探讨肿瘤干细胞标记物八聚体结合转录因子4(Oct4)和乙酰脱氢酶家族成员A1(ALDH1A1)在乳腺癌组织中的表达及其临床意义。方法采用免疫组织化学方法检测208例乳腺癌组织和80例乳腺腺瘤组织中的Oct4和ALDH1A1蛋白的表达,分析其表达与临床病理参数及预后的相关性,并分析二者表达的相关性;同时采用western blot法检测20例乳腺癌组织和20例乳腺腺瘤组织中Oct4和ALDH1A1蛋白的表达,并分析二者表达的相关性。结果Oct4和ALDH1A1蛋白在乳腺癌组织中的阳性表达明显高于乳腺腺瘤组织(P<0.05)。Oct4和ALDH1A1的表达与乳腺癌组织学分级、组织学类型、TNM分期、淋巴结转移及三阴性乳腺癌密切相关。Oct4和ALDH1A1高表达的患者预后明显差于低表达的患者(P<0.05)。乳腺癌组织中OCT4和ALDH1A1表达具有显著相关性(P<0.05)。结论乳腺癌组织中,Oct4和ALDH1A1表达与组织学分级、组织学类型、TNM分期、淋巴结转移及三阴性乳腺癌密切相关;Oct4和ALDH1A1高表达提示乳腺癌预后不良;OCT4和ALDH1A1表达具有相关性。
Objective To investigate the expression of tumor stem cell marker octamer binding transcription factor 4 (Oct4) and acetylcholine dehydrogenase family member A1 (ALDH1A1) in breast cancer and its clinical significance. Methods The expressions of Oct4 and ALDH1A1 protein in 208 cases of breast cancer and 80 cases of breast adenoma were detected by immunohistochemical method. The correlation between the expression of Oct4 and ALDH1A1 protein and clinical pathological parameters and prognosis was analyzed. Western blot was used to detect the expression of Oct4 and ALDH1A1 in 20 cases of breast cancer and 20 cases of breast adenoma. The correlation between them was analyzed. Results The positive expression of Oct4 and ALDH1A1 protein in breast cancer was significantly higher than that in breast adenoma (P <0.05). The expression of Oct4 and ALDH1A1 is closely related to histological grade, histological type, TNM stage, lymph node metastasis and triple negative breast cancer in breast cancer. Patients with high expression of Oct4 and ALDH1A1 had significantly worse prognosis than those with low expression (P <0.05). The expression of OCT4 and ALDH1A1 in breast cancer tissues was significantly correlated (P <0.05). Conclusions The expression of Oct4 and ALDH1A1 in breast cancer is closely related to the histological grade, histological type, TNM stage, lymph node metastasis and triple negative breast cancer. The high expression of Oct4 and ALDH1A1 suggests that the prognosis of breast cancer is poor. The expression of OCT4 and ALDH1A1 is correlated .