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本文报道用细胞培养方法,观察血卟啉衍生物(HPD)加激光照射对人体肝癌细胞的杀伤作用。实验结果显示,HPD(50μg/ml)对7721细胞的生长无影响,单独用激光(光距5.6cm,光斑直径3.8cm,功率21mW/cm~2)照射培养细胞20分钟,细胞的形态也无明显改变,但在培养液内加HPD后再用激光照射,细胞即可出现损伤,并随HPD浓度的增加和激光照射功率的加强,对7721人体肝癌细胞可见明显的杀伤作用。为了区分细胞损伤的程度,本文提出了细胞形态变化的分类法。 我们在实验中还发现:培养细胞在HPD加激光照射后,绝大多数细胞发生变性坏死,但其中尚有部分细胞存活而呈岛状分布,作者提出,这是由于激光束分布不均匀性所造成,并对过去HPD加红激光照射肿瘤的治疗中,仍有部分病例复发的原因提出了新的解释。因此,临床在用激光连续照射治疗肿瘤时,必须作顺序移动照射。
This article reported using cell culture methods to observe the killing effect of hematoporphyrin derivative (HPD) plus laser irradiation on human hepatoma cells. The experimental results showed that HPD (50 μg/ml) had no effect on the growth of 7721 cells. The cells were irradiated with laser light alone (distance from 5.6 cm, spot diameter of 3.8 cm, power of 21 mW/cm~2) for 20 minutes. Significant changes, but after adding HPD in the culture medium and then using laser irradiation, the cells can appear damage, and with the increase in the concentration of HPD and laser irradiation power to enhance the 7721 human hepatoma cells can significantly kill. In order to distinguish the degree of cell damage, this article proposes a classification of cell morphology changes. In our experiments, we also found that after HPD plus laser irradiation, most of the cells in the culture cells were degenerate and necrotic, but some of them survived and distributed in island form. The authors suggested that this is due to the uneven distribution of the laser beam. As a result, new explanations have been given to the causes of the recurrence of some cases of HPD plus red laser irradiation. Therefore, in the clinical treatment of tumors with continuous laser irradiation, it is necessary to perform sequential mobile irradiation.