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为观察不同病理类型原发性肾小球病患者的外周血中CD3、CD4和CD8三种T细胞亚群 ,及Th1和Th2所分泌的代表性细胞因子IL 4、IL 10和TNF α的变化 ,从而分别取原发性肾小球病患者的全血和血清 ,采用流式细胞技术测定T细胞亚群 ,ELISA法测定细胞因子IL 4、IL 10和TNF α。结果是原发性肾小球病组中CD3+ 、CD8+ 细胞比例增高 ,而不同类型的肾炎 ,T细胞亚群的变化不尽相同。微小病变型、系膜增生型和膜性肾病患者表现为IL 4增高 ,而IgA肾病患者则降低 ;四种不同的病理类型病人的IL 10均降低 ,尤以IgA肾病最明显。膜性肾病、微小病变型、系膜增生型肾炎患者Th2细胞功能增强 ;IgA肾病患者有Th1优势应答的可能 ;IL 10的水平明显降低可能是原发性肾小球病发生发展的重要机制之一
To observe the changes of peripheral blood CD3, CD4 and CD8 subsets and the expressions of IL-4, IL-10 and TNF-α secreted by Th1 and Th2 in patients with primary glomerulopathy with different pathological types , So as to take the whole blood and serum of patients with primary glomerulopathy separately. The T cell subsets were determined by flow cytometry. The cytokines IL 4, IL 10 and TNF α were determined by ELISA. The result is an increase in the proportion of CD3 + and CD8 + cells in the primary glomerulonephritis group, but not in the different types of nephritis and T cell subsets. Patients with minimal, mesangial, and membranous nephrons showed elevated IL-4 but decreased IgA nephropathy; IL-10 was reduced in all four different pathological types, most notably IgA nephropathy. Membranous nephropathy, minimal disease, and mesangial proliferative glomerulonephritis patients Th2 cell function enhancement; IgA nephropathy patients have the possibility of Th1 dominant response; IL 10 levels may be significantly reduced the incidence of primary glomerular disease is an important mechanism for the development of one