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采用毛细管区带电泳法 (CZE) ,以 5 0cm× 75 μmi d 毛细管柱作为分析柱 ,0 .0 2mol/L磷酸二氢钠 0 .0 2mol/L磷酸氢二钠混合体系 (pH 7.0 )作为背景电解质 ,以紫外检测器在波长 2 0 0nm的条件下检测 ,对具有生物活性的金属硫蛋白 (MT)的两种异构体 (MT1,MT2 )进行了分离。样品经过预处理后 ,采用外标法可对食品中的金属硫蛋白进行定量测定。该方法的最低检测质量浓度为 1mg/L ,相对标准偏差低于 10 % ,加标回收率为 82 .0 %～93.4%。
The capillary zone electrophoresis (CZE) was used to analyze the capillary column with a size of 50cm × 75μm, as the analytical column, and 0.02mol / L sodium dihydrogenphosphate0.02mol / L disodium hydrogenphosphate (pH7.0) The background electrolytes were detected with a UV detector at a wavelength of 200 nm, and the two isomers (MT1, MT2) of the biologically active metallothionein (MT) were isolated. After the sample is pre-treated, the external standard method can be used for the quantitative determination of metallothionein in food. The method of the lowest detection mass concentration of 1mg / L, the relative standard deviation of less than 10%, spiked recoveries of 82.0% to 93.4%.