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目的利用角膜缝线、碱烧伤两种常用方法来诱导兔角膜新生血管(CNV)产生。观察CNV产生早期的过程中,其生长情况及血管内皮生长因子(VEGF)在兔角膜中的表达情况。方法将新西兰白兔75只随机分为3组,A组-正常对照组5只;B组-角膜缝线组35只;C组-碱烧伤组35只。每日裂隙灯显微镜观察角膜炎性反应情况与混浊程度,检测第3天、7天、14天角膜新生血管长度和数量并摄影记录;B、C两组分别于实验后第1、2、3、5、7、10、14天观察、取材。免疫组织化学(IHC)检测VEGF在兔角膜中的表达情况。结果伤后3 d,新生血管开始侵入角膜;7 d,新生血管生长活跃;10 d,新生血管达到生长高峰;14 d后B组与C组新生血管均出现回退迹象。角膜缝线组CNV面积明显小于碱烧伤组(P<0.01),10 d后VEGF在碱烧伤组的表达明显高于缝线组(P<0.01)。结论碱烧伤组相对于缝线组可以诱导产生更多的新生血管(CNV面积),且CNV退化的时间晚,这些可能与VEGF的表达有关;但角膜缝线更容易控制CNV产生的范围。
Objective To use corneal suture and alkali burn to induce corneal neovascularization (CNV) in rabbits. The growth of CNV and the expression of vascular endothelial growth factor (VEGF) in rabbit cornea were observed during the early stage of CNV production. Methods 75 New Zealand white rabbits were randomly divided into 3 groups: group A - normal control group 5; group B - corneal suture group 35; group C - alkali burn group 35. Corneal inflammation and turbidity were observed daily by slit lamp microscope. The length and number of corneal neovascularization on day 3, 7 and 14 were detected and recorded. , 5,7,10,14 days observation, drawing. Immunohistochemistry (IHC) was used to detect the expression of VEGF in rabbit cornea. Results Neovascularization began to invade the cornea 3 days after injury. At 7 days, neovascularization was active. At 10 days, neovascularization reached the peak of growth. At 14 days, neovascularization showed signs of regression in both groups. The area of CNV in corneal suture group was significantly less than that in alkali burn group (P <0.01). After 10 days, the expression of VEGF in alkali burn group was significantly higher than that in suture group (P <0.01). Conclusions The alkali burn group can induce more neovascularization (CNV area) than the suture group, and the degeneration of CNV is late, which may be related to the expression of VEGF. However, the corneal suture can more easily control the range of CNV production.