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目的:观察独活寄生汤对椎间盘内紊乱(IDD)兔椎间盘组织中白细胞介素-1β(IL-1β)和前列腺素E2(PGE2)的影响,探讨独活寄生汤治疗盘源性腰痛的作用机制。方法:选取健康新西兰大白兔80只随机分为5组:正常组、模型组、低剂量组、中剂量组、高剂量组,每组16只。正常组不做任何干预,后4组进行手术造模,其中低、中、高剂量组在造模后进行独活寄生汤灌服治疗。各组兔子分别于4、8周时各处死8只,取髓核组织检测IL-1β和PGE2的含量。结果:与正常组相比,经手术造模的4组兔子椎间盘髓核组织中IL-1β、PGE2的含量明显升高(P<0.01,P<0.05);与模型组相比,高剂量组中IL-1β和PGE2的含量显著降低(P<0.05);治疗8周后,高剂量组中IL-1β和PGE2的含量与低、中剂量组相比均显著降低(P<0.01)。结论:独活寄生汤可以通过下调椎间盘髓核组织中IL-1β、PGE2的水平,延缓和抑制椎间盘退变,从而发挥其治疗椎间盘源性腰痛的作用。
Objective: To observe the effect of Dovestroke Decoction on interleukin-1β (IL-1β) and prostaglandin E2 (PGE2) in the intervertebral disc tissue of intervertebral disc disorder (IDD), and to explore the mechanism of Doveuzhi Decoction for treating low back pain. Methods: Eighty healthy New Zealand white rabbits were randomly divided into five groups: normal group, model group, low dose group, medium dose group and high dose group, with 16 rats in each group. The normal group did not make any intervention, the last 4 groups were operated on, and the low, medium and high dose groups were treated with the independent living parasitic soup after modeling. The rabbits in each group were sacrificed at the 4th and 8th week respectively, and the nucleus pulposus tissues were used to detect the contents of IL-1β and PGE2. Results: Compared with the normal group, the contents of IL-1β and PGE2 in the nucleus pulposus nucleus of the rabbits in the operation group were significantly increased (P <0.01, P <0.05). Compared with the model group, the high dose group The levels of IL-1β and PGE2 in the high-dose group were significantly lower than those in the low-dose and middle-dose groups (P <0.01) after 8 weeks of treatment. Conclusion: Dovestroke Decoction can delay and inhibit the degeneration of intervertebral disc by down-regulating the levels of IL-1β and PGE2 in the nucleus pulposus of the disc, thus playing its role in the treatment of discogenic low back pain.