【摘 要】
：
[目的]本实验旨在探究蜂花粉是否对高脂血症大鼠的肠道菌群具有调节作用.[方法]设置80、400和800 mg/kg 3个蜂花粉剂量组,另外设置高脂模型组和空白对照组,对高脂血症大鼠持
【机 构】
：
江西农业大学蜜蜂研究所,南昌330045;江西省蜜蜂生物学与饲养重点实验室,南昌330045;江西中医药大学实验动物科技中心,南昌330006
论文部分内容阅读
[目的]本实验旨在探究蜂花粉是否对高脂血症大鼠的肠道菌群具有调节作用.[方法]设置80、400和800 mg/kg 3个蜂花粉剂量组,另外设置高脂模型组和空白对照组,对高脂血症大鼠持续灌胃60 d后,利用16S rDNA测序技术测定大鼠直肠内容物中的菌群结构及多样性.[结果]高脂血症大鼠肠道中的细菌多样性相比空白对照组有下降趋势,且经过蜂花粉灌胃后并不能逆转这种趋势.从门水平上分析,5个组的大鼠肠道中厚壁菌门Firmicutes、拟杆菌门Bacteroidetes和疣微菌门Verrucomicrobia占总细菌的95.8％以上.从属水平上分析,与空白对照组相比,3个蜂花粉剂量组的大鼠肠道中拟杆菌属Bacteroides、粪球菌属Coprococcus和副普雷沃氏菌属Paraprevotella丰度显著下降(P＜0.05);与高脂模型组相比,3个蜂花粉组大鼠肠道中艾克曼菌属Akkermansia丰度上升(P＞0.05),而蜂花粉中剂量组大鼠肠道中拟杆菌属Bacteroides丰度显著上升(P＜0.05).[结论]蜂花粉对高脂血症大鼠肠道微生物结构具有一定的调节作用.
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