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目的观察对乙酰氨基酚联合外照射对脑胶质瘤SHG-44细胞株照射存活后代增殖的影响,探讨对乙酰氨基酚作为复发性恶性脑胶质瘤放射治疗增敏剂的可能性。方法培养人脑胶质瘤SHG-44细胞株经6 MV X线DT10 Gy照射后的存活后代细胞(SHG-44-10细胞),测定其群体倍增时间;在培养SHG-44-10细胞中加入对乙酰氨基酚,进行集落形成实验和流式细胞仪检测,分析其放射敏感性和细胞周期的变化。结果与SHG-44细胞相比较,SHG-44照射后代细胞克隆形成率降低,倍增时间延长,对放射的敏感性明显降低。而加入对乙酰氨基酚培养后,SHG-44照射后代细胞对放射的敏感性可增加。SHG-44照射后代细胞再次照射后12 h,G2/M相细胞比例增高,24 h比例下降,而加入对乙酰氨基酚培养后G2/M相细胞12 h、24 h均维持较高的比例。结论⑴SHG-44细胞照射后存活后代细胞增殖延缓,放射敏感性下降。⑵小剂量对乙酰氨基酚可提高SHG-44细胞照射后存活后代细胞的放射敏感性,其可能的机制为诱导细胞阻滞在对放射敏感的G2/M期并能诱导它的凋亡。⑶对乙酰氨基酚有可能成为治疗复发性脑胶质瘤的放射增敏剂。
Objective To investigate the effect of paracetamol combined with external irradiation on the proliferation of glioma SHG-44 cell line irradiated by irradiation and to explore the possibility of acetaminophen as radiosensitizer for recurrent malignant glioma. Methods Human glioma SHG-44 cells were cultured in SHG-44-10 cells after being irradiated with 6 MV X-ray DT10 Gy. The population doubling time was measured. SHG-44-10 cells Paracetamol, colony formation experiments and flow cytometry, analysis of its radiosensitivity and cell cycle changes. Results Compared with SHG-44 cells, SHG-44 irradiation generation of cloned cells reduced the rate of formation, prolongation of doubling time, the sensitivity of radiation was significantly reduced. After addition of acetaminophen, SHG-44 irradiated irradiation cells can increase the sensitivity of radiation. The percentage of G2 / M phase cells increased at 12 h after SHG-44 irradiation, and the proportion of G2 / M phase cells decreased after 24 h irradiation. G2 / M phase cells maintained high after 12 and 24 h of acetaminophen incubation. Conclusion ⑴ SHG-44 cells after irradiation survival of offspring proliferation slowed down, decreased radiosensitivity. (2) Low dose acetaminophen enhanced the radiosensitivity of surviving progenitor cells after irradiation with SHG-44 cells. The possible mechanism was that induced cell arrest in G2 / M phase sensitive to radiation and induced its apoptosis. Acetaminophen may become a radiosensitizer for the treatment of recurrent gliomas.